BMRB Entry 51101

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51101

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Title:
Backbone assignments of the heavy and light chains from the HLA-A*02:01/b2m/ELAGIGILTV(MART-1) human MHC-I protein complex
Deposition date:
2021-09-24
Original release date:
2023-06-15
Authors:
Truong, Hau; Sgourakis, Nikolaos
Citation:

Citation: Sun, Yi; Young, Michael; Woodward, Claire; Danon, Julia; Truong, Hau; Gupta, Sagar; Winters, Trenton; Font-Burgada, Joan; Burslem, George; Sgourakis, Nikolaos. "Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes"  Proc. Natl. Acad. Sci. U. S. A. 120, e2304055120-e2304055120 (2023).
PubMed: 37310998

Assembly members:

Assembly members:
entity_1, polymer, 277 residues, Formula weight is not available
entity_2, polymer, 100 residues, Formula weight is not available
entity_3, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSHSMRYFFTSVSRPGRGE PRFIAVGYVDDTQFVRFDSD AASQRMEPRAPWIEQEGPEY WDGETRKVKAHSQTHRVDLG TLRGYYNQSEAGSHTVQRMY GCDVGSDWRFLRGYHQYAYD GKDYIALKEDLRSWTAADMA AQTTKHKWEAAHVAEQLRAY LEGTCVEWLRRYLENGKETL QRTDAPKTHMTHHAVSDHEA TLRCWALSFYPAEITLTWQR DGEDQTQDTELVETRPAGDG TFQKWAAVVVPSGQEQRYTC HVQHEGLPKPLTLRWEP
entity_2: MIQRTPKIQVYSRHPAENGK SNFLNCYVSGFHPSDIEVDL LKNGERIEKVEHSDLSFSKD WSFYLLYYTEFTPTEKDEYA CRVNHVTLSQPKIVKWDRDM
entity_3: ELAGIGILTV

Data sets:
Data typeCount
13C chemical shifts794
15N chemical shifts304
1H chemical shifts304

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HLA-A*02:011
2Human Beta-2 microglobulin2
3MART-13

Entities:

Entity 1, HLA-A*02:01 277 residues - Formula weight is not available

1   METGLYSERHISSERMETARGTYRPHEPHE
2   THRSERVALSERARGPROGLYARGGLYGLU
3   PROARGPHEILEALAVALGLYTYRVALASP
4   ASPTHRGLNPHEVALARGPHEASPSERASP
5   ALAALASERGLNARGMETGLUPROARGALA
6   PROTRPILEGLUGLNGLUGLYPROGLUTYR
7   TRPASPGLYGLUTHRARGLYSVALLYSALA
8   HISSERGLNTHRHISARGVALASPLEUGLY
9   THRLEUARGGLYTYRTYRASNGLNSERGLU
10   ALAGLYSERHISTHRVALGLNARGMETTYR
11   GLYCYSASPVALGLYSERASPTRPARGPHE
12   LEUARGGLYTYRHISGLNTYRALATYRASP
13   GLYLYSASPTYRILEALALEULYSGLUASP
14   LEUARGSERTRPTHRALAALAASPMETALA
15   ALAGLNTHRTHRLYSHISLYSTRPGLUALA
16   ALAHISVALALAGLUGLNLEUARGALATYR
17   LEUGLUGLYTHRCYSVALGLUTRPLEUARG
18   ARGTYRLEUGLUASNGLYLYSGLUTHRLEU
19   GLNARGTHRASPALAPROLYSTHRHISMET
20   THRHISHISALAVALSERASPHISGLUALA
21   THRLEUARGCYSTRPALALEUSERPHETYR
22   PROALAGLUILETHRLEUTHRTRPGLNARG
23   ASPGLYGLUASPGLNTHRGLNASPTHRGLU
24   LEUVALGLUTHRARGPROALAGLYASPGLY
25   THRPHEGLNLYSTRPALAALAVALVALVAL
26   PROSERGLYGLNGLUGLNARGTYRTHRCYS
27   HISVALGLNHISGLUGLYLEUPROLYSPRO
28   LEUTHRLEUARGTRPGLUPRO

Entity 2, Human Beta-2 microglobulin 100 residues - Formula weight is not available

1   METILEGLNARGTHRPROLYSILEGLNVAL
2   TYRSERARGHISPROALAGLUASNGLYLYS
3   SERASNPHELEUASNCYSTYRVALSERGLY
4   PHEHISPROSERASPILEGLUVALASPLEU
5   LEULYSASNGLYGLUARGILEGLULYSVAL
6   GLUHISSERASPLEUSERPHESERLYSASP
7   TRPSERPHETYRLEULEUTYRTYRTHRGLU
8   PHETHRPROTHRGLULYSASPGLUTYRALA
9   CYSARGVALASNHISVALTHRLEUSERGLN
10   PROLYSILEVALLYSTRPASPARGASPMET

Entity 3, MART-1 10 residues - Formula weight is not available

1   GLULEUALAGLYILEGLYILELEUTHRVAL

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks