BMRB Entry 51102
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51102
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Title: TIA-1 prion-like domain, backbone chemical shifts PubMed: 36112647
Deposition date: 2021-09-26 Original release date: 2022-08-17
Authors: Sekiyama, Naotaka; Akagi, Ken-ichi; Kodama, Takashi; Tochio, Hidehito
Citation: Sekiyama, Naotaka; Takaba, Kiyofumi; Maki-Yonekura, Saori; Akagi, Ken-Ichi; Ohtani, Yasuko; Imamura, Kayo; Terakawa, Tsuyoshi; Yamashita, Keitaro; Inaoka, Daigo; Yonekura, Koji; Kodama, Takashi; Tochio, Hidehito. "ALS mutations in the TIA-1 prion-like domain trigger highly condensed pathogenic structures" Proc. Natl. Acad. Sci. U. S. A. 119, e2122523119-e2122523119 (2022).
Assembly members:
entity_1, polymer, 91 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHHHHHHHSENL
YFQGGQYVPNGWQVPAYGVY
GQPWSQQGFNQTQSSAPWMG
PNYSVPPPQGQNGSMLPSQP
AGYRVAGYETQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 207 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TIA-1 prion-like domain | 1 |
Entities:
Entity 1, TIA-1 prion-like domain 91 residues - Formula weight is not available
His-TEV-TIA1 320-386
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | HIS | HIS | HIS | HIS | HIS | SER | GLU | ASN | LEU | ||||
| 3 | TYR | PHE | GLN | GLY | GLY | GLN | TYR | VAL | PRO | ASN | ||||
| 4 | GLY | TRP | GLN | VAL | PRO | ALA | TYR | GLY | VAL | TYR | ||||
| 5 | GLY | GLN | PRO | TRP | SER | GLN | GLN | GLY | PHE | ASN | ||||
| 6 | GLN | THR | GLN | SER | SER | ALA | PRO | TRP | MET | GLY | ||||
| 7 | PRO | ASN | TYR | SER | VAL | PRO | PRO | PRO | GLN | GLY | ||||
| 8 | GLN | ASN | GLY | SER | MET | LEU | PRO | SER | GLN | PRO | ||||
| 9 | ALA | GLY | TYR | ARG | VAL | ALA | GLY | TYR | GLU | THR | ||||
| 10 | GLN |
Samples:
sample_1: TIA-1 prion-like domain, [U-99% 13C; U-99% 15N], 200 uM; MES 50 mM; sodium chloride 150 mM; DTT 1 mM; 1,6-hexanediol 10%
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
NMRFAM-SPARKY - data analysis
NMR spectrometers:
- Bruker AVANCE II 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts