BMRB Entry 51116
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51116
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Title: Backbone NMR resonance assignment of the disordered UBact protein from Nitrospira nitrosa PubMed: 35107780
Deposition date: 2021-09-30 Original release date: 2021-10-31
Authors: Bonn, Steven; Fushman, David
Citation: Bonn, Steven; Fushman, David. "Backbone NMR resonance assignment of the intrinsically disordered UBact protein from Nitrospira nitrosa" Biomol. NMR Assign. 16, 129-134 (2022).
Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available
Natural source: Common Name: Nitrospira nitrosa Taxonomy ID: 1742972 Superkingdom: Bacteria Kingdom: not available Genus/species: Nitrospira nitrosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-41a+
Entity Sequences (FASTA):
entity_1: MIQSLMPERRERPGDPMPKS
PSPLEEGGGPRRPETGSPDK
DSLLKRMRRVDPKQAERYRQ
RTGE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 165 |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 173 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBact | 1 |
Entities:
Entity 1, UBact 64 residues - Formula weight is not available
| 1 | MET | ILE | GLN | SER | LEU | MET | PRO | GLU | ARG | ARG | ||||
| 2 | GLU | ARG | PRO | GLY | ASP | PRO | MET | PRO | LYS | SER | ||||
| 3 | PRO | SER | PRO | LEU | GLU | GLU | GLY | GLY | GLY | PRO | ||||
| 4 | ARG | ARG | PRO | GLU | THR | GLY | SER | PRO | ASP | LYS | ||||
| 5 | ASP | SER | LEU | LEU | LYS | ARG | MET | ARG | ARG | VAL | ||||
| 6 | ASP | PRO | LYS | GLN | ALA | GLU | ARG | TYR | ARG | GLN | ||||
| 7 | ARG | THR | GLY | GLU |
Samples:
sample_1: UBact, [U-98% 13C; U-98% 15N], 500 uM; sodium phosphate 20 mM; D2O 5%
sample_conditions_1: ionic strength: 36 mM; pH: 6.8; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.0.8 - collection, processing
CcpNMR v2.4.2 - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Related Database Links:
| NCBI | WP_090742413 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts