BMRB Entry 51123

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51123

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Title:
1H, 15N, 13C backbone chemical shift assignment of ApoE4-NTD at pH 3.5 20C
Deposition date:
2021-10-03
Original release date:
2021-11-28
Authors:
Pandit, Subhendu
Citation:

Citation: Pandit, Subhendu. "1H, 15N and 13C chemical shift assignments of the N-terminal domain of the two isoforms of the human apolipoprotein E"  Biomol. NMR Assign. 16, 191-196 (2022).
PubMed: 35451799

Assembly members:

Assembly members:
entity_1, polymer, 167 residues, 18622.05 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KVEQAVETEPEPELRQQTEW QSGQRWELALGRFWDYLRWV QTLSEQVQEELLSSQVTQEL RALMDETMKELKAYKSELEE QLTPVAEETRARLSKELQAA QARLGADMEDVRGRLVQYRG EVQAMLGQSTEELRVRLASH LRKLRKRLLRDADDLQKRLA VYQAGAR

Data sets:
Data typeCount
13C chemical shifts613
15N chemical shifts154
1H chemical shifts775

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ApoE4-NTD1

Entities:

Entity 1, ApoE4-NTD 167 residues - 18622.05 Da.

1   LYSVALGLUGLNALAVALGLUTHRGLUPRO
2   GLUPROGLULEUARGGLNGLNTHRGLUTRP
3   GLNSERGLYGLNARGTRPGLULEUALALEU
4   GLYARGPHETRPASPTYRLEUARGTRPVAL
5   GLNTHRLEUSERGLUGLNVALGLNGLUGLU
6   LEULEUSERSERGLNVALTHRGLNGLULEU
7   ARGALALEUMETASPGLUTHRMETLYSGLU
8   LEULYSALATYRLYSSERGLULEUGLUGLU
9   GLNLEUTHRPROVALALAGLUGLUTHRARG
10   ALAARGLEUSERLYSGLULEUGLNALAALA
11   GLNALAARGLEUGLYALAASPMETGLUASP
12   VALARGGLYARGLEUVALGLNTYRARGGLY
13   GLUVALGLNALAMETLEUGLYGLNSERTHR
14   GLUGLULEUARGVALARGLEUALASERHIS
15   LEUARGLYSLEUARGLYSARGLEULEUARG
16   ASPALAASPASPLEUGLNLYSARGLEUALA
17   VALTYRGLNALAGLYALAARG

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks