BMRB Entry 51133
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51133
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Title: Backbone 1H, 13C and 15N Chemical Shift Assignments for Stress Granule Key Component G3BP1 RRM domain PubMed: 35150414
Deposition date: 2021-10-12 Original release date: 2022-02-18
Authors: Zhang, Hanyu; Tu, Xiaoming; Zhang, Jiahai
Citation: Zhang, Hanyu; Tu, Xiaoming; Zhang, Jiahai. "1H, 13C and 15N resonance assignments of stress granule key component G3BP1 RRM domain" Biomol. NMR Assignments 16, 109-111 (2022).
Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28A
Entity Sequences (FASTA):
entity_1: GSSHHHHHHSSGENLYFQHM
DIEPRRMVRHPDSHQLFIGN
LPHEVDKSELKDFFQSYGNV
VELRINSGGKLPNFGFVVFD
DSEPVQKVLSNRPIMFRGEV
RLNVEEKKTRAAREGDRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 265 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 183 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | G3BP1 RRM | 1 |
Entities:
Entity 1, G3BP1 RRM 118 residues - Formula weight is not available
The first 20 amino acids are His-Tag and the last 98 are RRM Domian.
| 1 | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | SER | ||||
| 2 | SER | GLY | GLU | ASN | LEU | TYR | PHE | GLN | HIS | MET | ||||
| 3 | ASP | ILE | GLU | PRO | ARG | ARG | MET | VAL | ARG | HIS | ||||
| 4 | PRO | ASP | SER | HIS | GLN | LEU | PHE | ILE | GLY | ASN | ||||
| 5 | LEU | PRO | HIS | GLU | VAL | ASP | LYS | SER | GLU | LEU | ||||
| 6 | LYS | ASP | PHE | PHE | GLN | SER | TYR | GLY | ASN | VAL | ||||
| 7 | VAL | GLU | LEU | ARG | ILE | ASN | SER | GLY | GLY | LYS | ||||
| 8 | LEU | PRO | ASN | PHE | GLY | PHE | VAL | VAL | PHE | ASP | ||||
| 9 | ASP | SER | GLU | PRO | VAL | GLN | LYS | VAL | LEU | SER | ||||
| 10 | ASN | ARG | PRO | ILE | MET | PHE | ARG | GLY | GLU | VAL | ||||
| 11 | ARG | LEU | ASN | VAL | GLU | GLU | LYS | LYS | THR | ARG | ||||
| 12 | ALA | ALA | ARG | GLU | GLY | ASP | ARG | ARG |
Samples:
sample_1: D2O 10%; sodium phosphate 0.02 M; sodium chloride 0.15 M; G3BP1 RRM domain, [U-99% 15N], 0.5 mM
sample_conditions_1: ionic strength: 0.27 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HACANH | sample_1 | isotropic | sample_conditions_1 |
| 3D HACACONH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - peak picking
CARA - chemical shift assignment
NMRPipe - processing
NMR spectrometers:
- Agilent INOVA 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts