BMRB Entry 51134
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51134
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Title: pre-miRNA-31 BottomB PubMed: 35114594
Deposition date: 2021-10-12 Original release date: 2022-02-18
Authors: Kotar, Anita; Keane, Sarah
Citation: Kotar, Anita; Ma, Sicong; Keane, Sarah. "pH dependence of C*A, G*A and A*A mismatches in the stem of precursor microRNA-31" Biophys. Chem. 283, 106763-106763 (2022).
Assembly members:
entity_1, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: in vitro enzymatic synthesis
Entity Sequences (FASTA):
entity_1: GGCAAGAUGCUGGGAGACCA
ACAUAUUGCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 38 |
| 1H chemical shifts | 141 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA | 1 |
Entities:
Entity 1, RNA 30 residues - Formula weight is not available
| 1 | G | G | C | A | A | G | A | U | G | C | |
| 2 | U | G | G | G | A | G | A | C | C | A | |
| 3 | A | C | A | U | A | U | U | G | C | C |
Samples:
sample_1: RNA 0.4 mM; D2O 100%; potassium phosphate 50 mM; magnesium chloride 1 mM
sample_conditions_1: ionic strength: 0.2255 M; pH: 7.5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ v9.2.0-b24 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
- Bruker AVANCE NEO 600 MHz