BMRB Entry 51143

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51143

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Title:
H3 tail in 193-bp nucleosome
Deposition date:
2021-10-14
Original release date:
2023-03-10
Authors:
Ohtomo, Hideaki
Citation:

Citation: Ohtomo, Hideaki; Ito, Shinsuke; McKenzie, Nicholas; Uckelmann, Michael; Wakamori, Masatoshi; Ehara, Haruhiko; Furukawa, Ayako; Tsunaka, Yasuo; Shibata, Marika; Sekine, Shun-Ichi; Umehara, Takashi; Davidovich, Chen; Koseki, Haruhiko; Nishimura, Yoshifumi. "H2A Ubiquitination Alters H3-tail Dynamics on Linker-DNA to Enhance H3K27 Methylation"  J. Mol. Biol. 435, 167936-167936 (2023).
PubMed: 36610636

Assembly members:

Assembly members:
entity_1, polymer, 135 residues, Formula weight is not available
entity_2, polymer, 133 residues, Formula weight is not available
entity_3, polymer, 129 residues, Formula weight is not available
entity_4, polymer, 102 residues, Formula weight is not available
entity_5, polymer, 76 residues, Formula weight is not available
entity_6, polymer, 193 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ARTKQTARKSTGGKAPRKQL ATKAARKSAPATGGVKKPHR YRPGTVALREIRRYQKSTEL LIRKLPFQRLVREIAQDFKT DLRFQSSAVMALQEACEAYL VGLFEDTNLCAIHAKRVTIM PKDIQLARRIRGERA
entity_2: GPGMSGRGKQGGKARAKAKT RSSRAGLQFPVGRVHRLLRK GNYSERVGAGAPVYLAAVLE YLTAEILELAGNAARDNKKT RIIPRHLQLAIRNDEELNKL LGRVTIAQGGVLPNIQAVLL PKKTESHHKAKGK
entity_3: GPGMPEPAKSAPAPKKGSKK AVTKAQKKDGKKRKRSRKES YSIYVYKVLKQVHPDTGISS KAMGIMNSFVNDIFERIAGE ASRLAHYNKRSTITSREIQT AVRLLLPGELAKHAVSEGTK AVTKYTSAK
entity_4: SGRGKGGKGLGKGGAKRHRK VLRDNIQGITKPAIRRLARR GGVKRISGLIYEETRGVLKV FLENVIRDAVTYTEHAKRKT VTAMDVVYALKRQGRTLYGF GG
entity_5: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG
entity_6: ATCGGACCCTATCGCGAGCC AGGCCTGAGAATCCGGTGCC GAGGCCGCTCAATTGGTCGT AGACAGCTCTAGCACCGCTT AAACGCACGTACGCGCTGTC CCCCGCGTTTTAACCGCCAA GGGGATTACTCCCTAGTCTC CAGGCACGTGTCAGATATAT ACATCCAGGCCTTGTGTCGC GAAATTCATAGAT

Data sets:
Data typeCount
15N chemical shifts43
1H chemical shifts43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1nucleosome, H31
2nucleosome, H2A2
3nucleosome, H2B3
4nucleosome, H44
5ubiquitin5
6DNA6

Entities:

Entity 1, nucleosome, H3 135 residues - Formula weight is not available

1   ALAARGTHRLYSGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU
3   ALATHRLYSALAALAARGLYSSERALAPRO
4   ALATHRGLYGLYVALLYSLYSPROHISARG
5   TYRARGPROGLYTHRVALALALEUARGGLU
6   ILEARGARGTYRGLNLYSSERTHRGLULEU
7   LEUILEARGLYSLEUPROPHEGLNARGLEU
8   VALARGGLUILEALAGLNASPPHELYSTHR
9   ASPLEUARGPHEGLNSERSERALAVALMET
10   ALALEUGLNGLUALACYSGLUALATYRLEU
11   VALGLYLEUPHEGLUASPTHRASNLEUCYS
12   ALAILEHISALALYSARGVALTHRILEMET
13   PROLYSASPILEGLNLEUALAARGARGILE
14   ARGGLYGLUARGALA

Entity 2, nucleosome, H2A 133 residues - Formula weight is not available

1   GLYPROGLYMETSERGLYARGGLYLYSGLN
2   GLYGLYLYSALAARGALALYSALALYSTHR
3   ARGSERSERARGALAGLYLEUGLNPHEPRO
4   VALGLYARGVALHISARGLEULEUARGLYS
5   GLYASNTYRSERGLUARGVALGLYALAGLY
6   ALAPROVALTYRLEUALAALAVALLEUGLU
7   TYRLEUTHRALAGLUILELEUGLULEUALA
8   GLYASNALAALAARGASPASNLYSLYSTHR
9   ARGILEILEPROARGHISLEUGLNLEUALA
10   ILEARGASNASPGLUGLULEUASNLYSLEU
11   LEUGLYARGVALTHRILEALAGLNGLYGLY
12   VALLEUPROASNILEGLNALAVALLEULEU
13   PROLYSLYSTHRGLUSERHISHISLYSALA
14   LYSGLYLYS

Entity 3, nucleosome, H2B 129 residues - Formula weight is not available

1   GLYPROGLYMETPROGLUPROALALYSSER
2   ALAPROALAPROLYSLYSGLYSERLYSLYS
3   ALAVALTHRLYSALAGLNLYSLYSASPGLY
4   LYSLYSARGLYSARGSERARGLYSGLUSER
5   TYRSERILETYRVALTYRLYSVALLEULYS
6   GLNVALHISPROASPTHRGLYILESERSER
7   LYSALAMETGLYILEMETASNSERPHEVAL
8   ASNASPILEPHEGLUARGILEALAGLYGLU
9   ALASERARGLEUALAHISTYRASNLYSARG
10   SERTHRILETHRSERARGGLUILEGLNTHR
11   ALAVALARGLEULEULEUPROGLYGLULEU
12   ALALYSHISALAVALSERGLUGLYTHRLYS
13   ALAVALTHRLYSTYRTHRSERALALYS

Entity 4, nucleosome, H4 102 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSGLYLEU
2   GLYLYSGLYGLYALALYSARGHISARGLYS
3   VALLEUARGASPASNILEGLNGLYILETHR
4   LYSPROALAILEARGARGLEUALAARGARG
5   GLYGLYVALLYSARGILESERGLYLEUILE
6   TYRGLUGLUTHRARGGLYVALLEULYSVAL
7   PHELEUGLUASNVALILEARGASPALAVAL
8   THRTYRTHRGLUHISALALYSARGLYSTHR
9   VALTHRALAMETASPVALVALTYRALALEU
10   LYSARGGLNGLYARGTHRLEUTYRGLYPHE
11   GLYGLY

Entity 5, ubiquitin 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Entity 6, DNA 193 residues - Formula weight is not available

1   DADTDCDGDGDADCDCDCDT
2   DADTDCDGDCDGDADGDCDC
3   DADGDGDCDCDTDGDADGDA
4   DADTDCDCDGDGDTDGDCDC
5   DGDADGDGDCDCDGDCDTDC
6   DADADTDTDGDGDTDCDGDT
7   DADGDADCDADGDCDTDCDT
8   DADGDCDADCDCDGDCDTDT
9   DADADADCDGDCDADCDGDT
10   DADCDGDCDGDCDTDGDTDC
11   DCDCDCDCDGDCDGDTDTDT
12   DTDADADCDCDGDCDCDADA
13   DGDGDGDGDADTDTDADCDT
14   DCDCDCDTDADGDTDCDTDC
15   DCDADGDGDCDADCDGDTDG
16   DTDCDADGDADTDADTDADT
17   DADCDADTDCDCDADGDGDC
18   DCDTDTDGDTDGDTDCDGDC
19   DGDADADADTDTDCDADTDA
20   DGDADT

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks