BMRB Entry 51155

Name: Unmodified siRNA
Published: 2022-03-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51155

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3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Unmodified siRNA PubMed: 35263184

Deposition date: 2021-10-25 Original release date: 2022-03-29

Authors: Becette, Owen; Tran, Anh; Jones, Jace; Marino, John; Brinson, Robert

Citation: Becette, Owen; Tran, Anh; Jones, Jace; Marino, John; Brinson, Robert. "Structural Fingerprinting of Short Interfering RNA Therapeutics by Solution Nuclear Magnetic Resonance Spectroscopy" Nucleic Acid Ther. 32, 267-279 (2022).

Assembly members:
entity_1, polymer, 17 residues, Formula weight is not available
entity_2, polymer, 17 residues, Formula weight is not available

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GCAUAGCACUGUUCGTT
entity_2: CGAACAGUGCUAUGCTT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	97
15N chemical shifts	15
1H chemical shifts	238

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sense siRNA	1
2	Antisense siRNA	2

Entities:

Entity 1, Sense siRNA 17 residues - Formula weight is not available

Antisense strand of the unmodified siRNA

1

G

C

A

U

A

G

C

A

C

U

2

G

U

C

G

DT

Entity 2, Antisense siRNA 17 residues - Formula weight is not available

Sense strand of the unmodified siRNA

1

C

G

A

C

A

G

U

G

C

2

U

A

U

G

C

DT

Samples:

sample_1: Sense siRNA 2 mM; Antisense siRNA 2 mM; sodium phosphate 10 mM; sodium chloride 50 mM; sodium azide 0.02%; D2O, [U-99% 2H], 5%

sample_2: Sense siRNA 2 mM; Antisense siRNA 2 mM; sodium phosphate 10 mM; sodium chloride 50 mM; sodium azide 0.02%; D2O, [U-99% 2H], 100%

sample_conditions_1: pH: 6.5; temperature: 283 K

sample_conditions_2: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-13C HMQC	sample_2	isotropic	sample_conditions_2
2D 1H-13C HMQC	sample_2	isotropic	sample_conditions_2
2D 1H-13C HMQC	sample_2	isotropic	sample_conditions_2
2D 1H-13C HMQC	sample_2	isotropic	sample_conditions_2

Software:

NMRViewJ - data analysis

NMRPipe - processing

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE III 900 MHz