BMRB Entry 51159
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51159
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Title: Backbone chemical shift assignments of Horcolin PubMed: 35225598
Deposition date: 2021-10-28 Original release date: 2022-03-10
Authors: Narayanan, Vaishali; Bobbili, Kishore Babu; Sivaji, Nukathoti; Jayaprakash, Nisha G; Suguna, Kaza; Surolia, Avadesha; Sekhar, Ashok
Citation: Narayanan, Vaishali; Bobbili, Kishore Babu; Sivaji, Nukathoti; Jayaprakash, Nisha G; Suguna, Kaza; Surolia, Avadesha; Sekhar, Ashok. "Structure and Carbohydrate Recognition by the Nonmitogenic Lectin Horcolin" Biochemistry 61, 464-478 (2022).
Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available
Natural source: Common Name: Barley Taxonomy ID: 4513 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Hordeum vulgare
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b(+)
Entity Sequences (FASTA):
entity_1: MSKPVKIGPWGGNGGSERDV
QPKPIRMVSMTVSSGAIVDA
IAFTYVGTDNVQHSSGIKWG
GTGGTEDTINLDATNYVTEI
SGTVGKFGTDDIVTSLKIIT
SKGVTRTYGSGTGIPFRVPV
LDGGKIAGFFGRAGAFLDAI
GFYITP
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 387 |
15N chemical shifts | 130 |
1H chemical shifts | 285 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Horcolin, subunit 1 | 1 |
2 | Horcolin, subunit 2 | 1 |
Entities:
Entity 1, Horcolin, subunit 1 146 residues - Formula weight is not available
1 | MET | SER | LYS | PRO | VAL | LYS | ILE | GLY | PRO | TRP | ||||
2 | GLY | GLY | ASN | GLY | GLY | SER | GLU | ARG | ASP | VAL | ||||
3 | GLN | PRO | LYS | PRO | ILE | ARG | MET | VAL | SER | MET | ||||
4 | THR | VAL | SER | SER | GLY | ALA | ILE | VAL | ASP | ALA | ||||
5 | ILE | ALA | PHE | THR | TYR | VAL | GLY | THR | ASP | ASN | ||||
6 | VAL | GLN | HIS | SER | SER | GLY | ILE | LYS | TRP | GLY | ||||
7 | GLY | THR | GLY | GLY | THR | GLU | ASP | THR | ILE | ASN | ||||
8 | LEU | ASP | ALA | THR | ASN | TYR | VAL | THR | GLU | ILE | ||||
9 | SER | GLY | THR | VAL | GLY | LYS | PHE | GLY | THR | ASP | ||||
10 | ASP | ILE | VAL | THR | SER | LEU | LYS | ILE | ILE | THR | ||||
11 | SER | LYS | GLY | VAL | THR | ARG | THR | TYR | GLY | SER | ||||
12 | GLY | THR | GLY | ILE | PRO | PHE | ARG | VAL | PRO | VAL | ||||
13 | LEU | ASP | GLY | GLY | LYS | ILE | ALA | GLY | PHE | PHE | ||||
14 | GLY | ARG | ALA | GLY | ALA | PHE | LEU | ASP | ALA | ILE | ||||
15 | GLY | PHE | TYR | ILE | THR | PRO |
Samples:
sample_1: Horcolin, [U-100% 15N], 1 mM; D2O, [U-2H], 10 % v/v; H2O 90 % v/v; TRIS 25 mM; sodium chloride 50 mM; EDTA 1 mM; sodium azide 0.03 % w/v
sample_2: Horcolin, [U-99% 13C; U-99% 15N], 1 mM; D2O, [U-2H], 10 % v/v; H2O 90 % v/v; TRIS 25 mM; sodium chloride 50 mM; EDTA 1 mM; sodium azide 0.03 % w/v
sample_3: Horcolin, [U-13C; U-15N; U-2H], 0.6 mM; D2O, [U-2H], 10 % v/v; H2O 90 % v/v; TRIS 25 mM; sodium chloride 50 mM; EDTA 1 mM; sodium azide 0.03 % w/v
sample_conditions_1: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCO | sample_3 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_3 | isotropic | sample_conditions_1 |
3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_3 | isotropic | sample_conditions_1 |
3D HNN | sample_3 | isotropic | sample_conditions_1 |
3D HNCA | sample_2 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D HACA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
SPARKY - chemical shift assignment, data analysis, peak picking
TALOS+ - geometry optimization
NMR spectrometers:
- Agilent DRX 600 MHz
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts