BMRB Entry 51159

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51159

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Title:
Backbone chemical shift assignments of Horcolin
Deposition date:
2021-10-28
Original release date:
2022-03-10
Authors:
Narayanan, Vaishali; Bobbili, Kishore Babu; Sivaji, Nukathoti; Jayaprakash, Nisha G; Suguna, Kaza; Surolia, Avadesha; Sekhar, Ashok
Citation:

Citation: Narayanan, Vaishali; Bobbili, Kishore Babu; Sivaji, Nukathoti; Jayaprakash, Nisha G; Suguna, Kaza; Surolia, Avadesha; Sekhar, Ashok. "Structure and Carbohydrate Recognition by the Nonmitogenic Lectin Horcolin"  Biochemistry 61, 464-478 (2022).
PubMed: 35225598

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Barley   Taxonomy ID: 4513   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Hordeum vulgare

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSKPVKIGPWGGNGGSERDV QPKPIRMVSMTVSSGAIVDA IAFTYVGTDNVQHSSGIKWG GTGGTEDTINLDATNYVTEI SGTVGKFGTDDIVTSLKIIT SKGVTRTYGSGTGIPFRVPV LDGGKIAGFFGRAGAFLDAI GFYITP

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts130
1H chemical shifts285

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Horcolin, subunit 11
2Horcolin, subunit 21

Entities:

Entity 1, Horcolin, subunit 1 146 residues - Formula weight is not available

1   METSERLYSPROVALLYSILEGLYPROTRP
2   GLYGLYASNGLYGLYSERGLUARGASPVAL
3   GLNPROLYSPROILEARGMETVALSERMET
4   THRVALSERSERGLYALAILEVALASPALA
5   ILEALAPHETHRTYRVALGLYTHRASPASN
6   VALGLNHISSERSERGLYILELYSTRPGLY
7   GLYTHRGLYGLYTHRGLUASPTHRILEASN
8   LEUASPALATHRASNTYRVALTHRGLUILE
9   SERGLYTHRVALGLYLYSPHEGLYTHRASP
10   ASPILEVALTHRSERLEULYSILEILETHR
11   SERLYSGLYVALTHRARGTHRTYRGLYSER
12   GLYTHRGLYILEPROPHEARGVALPROVAL
13   LEUASPGLYGLYLYSILEALAGLYPHEPHE
14   GLYARGALAGLYALAPHELEUASPALAILE
15   GLYPHETYRILETHRPRO

Related Database Links:

UNP Q5U9T2
AlphaFold Q5U9T2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks