BMRB Entry 51162
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51162
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Title: N88R CtRNHI PubMed: 35312304
Deposition date: 2021-10-30 Original release date: 2022-03-28
Authors: Martin, James; Palmer, Arthur
Citation: Martin, James; Palmer, Arthur. "Comparisons of Ribonuclease HI Homologs and Mutants Uncover a Multistate Model for Substrate Recognition" J. Am. Chem. Soc. 144, 5342-5349 (2022).
Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available
Natural source: Common Name: Clostridium tepidum Taxonomy ID: 1962263 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium tepidum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-25b(+)
Entity Sequences (FASTA):
entity_1: MEKTITIYTDGAASGNPGKG
GWGALLMYGSSRKEISGYDP
ATTNNRMELMAAIKGLEALK
EPARVQLYSDSAYLVNAMNE
GWLKRWVKRGWKTAAKKPVE
NIDLWQEILKLTTLHRVTFH
KVKGHSDNPYNSRADELARL
AIKENS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 145 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N88R CtRNHI | 1 |
Entities:
Entity 1, N88R CtRNHI 146 residues - Formula weight is not available
| 1 | MET | GLU | LYS | THR | ILE | THR | ILE | TYR | THR | ASP | ||||
| 2 | GLY | ALA | ALA | SER | GLY | ASN | PRO | GLY | LYS | GLY | ||||
| 3 | GLY | TRP | GLY | ALA | LEU | LEU | MET | TYR | GLY | SER | ||||
| 4 | SER | ARG | LYS | GLU | ILE | SER | GLY | TYR | ASP | PRO | ||||
| 5 | ALA | THR | THR | ASN | ASN | ARG | MET | GLU | LEU | MET | ||||
| 6 | ALA | ALA | ILE | LYS | GLY | LEU | GLU | ALA | LEU | LYS | ||||
| 7 | GLU | PRO | ALA | ARG | VAL | GLN | LEU | TYR | SER | ASP | ||||
| 8 | SER | ALA | TYR | LEU | VAL | ASN | ALA | MET | ASN | GLU | ||||
| 9 | GLY | TRP | LEU | LYS | ARG | TRP | VAL | LYS | ARG | GLY | ||||
| 10 | TRP | LYS | THR | ALA | ALA | LYS | LYS | PRO | VAL | GLU | ||||
| 11 | ASN | ILE | ASP | LEU | TRP | GLN | GLU | ILE | LEU | LYS | ||||
| 12 | LEU | THR | THR | LEU | HIS | ARG | VAL | THR | PHE | HIS | ||||
| 13 | LYS | VAL | LYS | GLY | HIS | SER | ASP | ASN | PRO | TYR | ||||
| 14 | ASN | SER | ARG | ALA | ASP | GLU | LEU | ALA | ARG | LEU | ||||
| 15 | ALA | ILE | LYS | GLU | ASN | SER |
Samples:
sample_1: V98A E. coli Ribonuclease HI, [U-100% 13C; U-100% 15N], 325 uM; deuterated sodium acetate 50 mM; sodium chloride 50 mM; sodium azide 0.02 % (w/v); DSS 3 mM
sample_conditions_1: ionic strength: 0.050 M; pH: 5.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts