BMRB Entry 51162

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51162

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Title:
N88R CtRNHI
Deposition date:
2021-10-30
Original release date:
2022-03-28
Authors:
Martin, James; Palmer, Arthur
Citation:

Citation: Martin, James; Palmer, Arthur. "Comparisons of Ribonuclease HI Homologs and Mutants Uncover a Multistate Model for Substrate Recognition"  J. Am. Chem. Soc. 144, 5342-5349 (2022).
PubMed: 35312304

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Clostridium tepidum   Taxonomy ID: 1962263   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium tepidum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-25b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEKTITIYTDGAASGNPGKG GWGALLMYGSSRKEISGYDP ATTNNRMELMAAIKGLEALK EPARVQLYSDSAYLVNAMNE GWLKRWVKRGWKTAAKKPVE NIDLWQEILKLTTLHRVTFH KVKGHSDNPYNSRADELARL AIKENS

Data sets:
Data typeCount
15N chemical shifts145
1H chemical shifts145

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1N88R CtRNHI1

Entities:

Entity 1, N88R CtRNHI 146 residues - Formula weight is not available

1   METGLULYSTHRILETHRILETYRTHRASP
2   GLYALAALASERGLYASNPROGLYLYSGLY
3   GLYTRPGLYALALEULEUMETTYRGLYSER
4   SERARGLYSGLUILESERGLYTYRASPPRO
5   ALATHRTHRASNASNARGMETGLULEUMET
6   ALAALAILELYSGLYLEUGLUALALEULYS
7   GLUPROALAARGVALGLNLEUTYRSERASP
8   SERALATYRLEUVALASNALAMETASNGLU
9   GLYTRPLEULYSARGTRPVALLYSARGGLY
10   TRPLYSTHRALAALALYSLYSPROVALGLU
11   ASNILEASPLEUTRPGLNGLUILELEULYS
12   LEUTHRTHRLEUHISARGVALTHRPHEHIS
13   LYSVALLYSGLYHISSERASPASNPROTYR
14   ASNSERARGALAASPGLULEUALAARGLEU
15   ALAILELYSGLUASNSER

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SPARKY: Backbone or all simulated peaks