BMRB Entry 51163

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51163

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Title:
R88N EcRNHI
Deposition date:
2021-10-30
Original release date:
2022-03-28
Authors:
Martin, James; Palmer, Arthur
Citation:

Citation: Martin, James; Palmer, Arthur. "Comparisons of Ribonuclease HI Homologs and Mutants Uncover a Multistate Model for Substrate Recognition"  J. Am. Chem. Soc. 144, 5342-5349 (2022).
PubMed: 35312304

Assembly members:

Assembly members:
entity_1, polymer, 155 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-25b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MLKQVEIFTDGSCLGNPGPG GYGAILRYRGREKTFSAGYT RTTNNRMELMAAIVALEALK EHCEVILSTDSQYVRQGITQ WIHNWKKNGWKTADKKPVKN VDLWQRLDAALGQHQIKWEW VKGHAGHPENERCDELARAA AMNPTLEDTGYQVEV

Data sets:
Data typeCount
15N chemical shifts158
1H chemical shifts158

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1R88N EcRNHI1

Entities:

Entity 1, R88N EcRNHI 155 residues - Formula weight is not available

1   METLEULYSGLNVALGLUILEPHETHRASP
2   GLYSERCYSLEUGLYASNPROGLYPROGLY
3   GLYTYRGLYALAILELEUARGTYRARGGLY
4   ARGGLULYSTHRPHESERALAGLYTYRTHR
5   ARGTHRTHRASNASNARGMETGLULEUMET
6   ALAALAILEVALALALEUGLUALALEULYS
7   GLUHISCYSGLUVALILELEUSERTHRASP
8   SERGLNTYRVALARGGLNGLYILETHRGLN
9   TRPILEHISASNTRPLYSLYSASNGLYTRP
10   LYSTHRALAASPLYSLYSPROVALLYSASN
11   VALASPLEUTRPGLNARGLEUASPALAALA
12   LEUGLYGLNHISGLNILELYSTRPGLUTRP
13   VALLYSGLYHISALAGLYHISPROGLUASN
14   GLUARGCYSASPGLULEUALAARGALAALA
15   ALAMETASNPROTHRLEUGLUASPTHRGLY
16   TYRGLNVALGLUVAL

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks