BMRB Entry 51163
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51163
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Title: R88N EcRNHI PubMed: 35312304
Deposition date: 2021-10-30 Original release date: 2022-03-28
Authors: Martin, James; Palmer, Arthur
Citation: Martin, James; Palmer, Arthur. "Comparisons of Ribonuclease HI Homologs and Mutants Uncover a Multistate Model for Substrate Recognition" J. Am. Chem. Soc. 144, 5342-5349 (2022).
Assembly members:
entity_1, polymer, 155 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-25b(+)
Entity Sequences (FASTA):
entity_1: MLKQVEIFTDGSCLGNPGPG
GYGAILRYRGREKTFSAGYT
RTTNNRMELMAAIVALEALK
EHCEVILSTDSQYVRQGITQ
WIHNWKKNGWKTADKKPVKN
VDLWQRLDAALGQHQIKWEW
VKGHAGHPENERCDELARAA
AMNPTLEDTGYQVEV
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 158 |
| 1H chemical shifts | 158 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | R88N EcRNHI | 1 |
Entities:
Entity 1, R88N EcRNHI 155 residues - Formula weight is not available
| 1 | MET | LEU | LYS | GLN | VAL | GLU | ILE | PHE | THR | ASP | ||||
| 2 | GLY | SER | CYS | LEU | GLY | ASN | PRO | GLY | PRO | GLY | ||||
| 3 | GLY | TYR | GLY | ALA | ILE | LEU | ARG | TYR | ARG | GLY | ||||
| 4 | ARG | GLU | LYS | THR | PHE | SER | ALA | GLY | TYR | THR | ||||
| 5 | ARG | THR | THR | ASN | ASN | ARG | MET | GLU | LEU | MET | ||||
| 6 | ALA | ALA | ILE | VAL | ALA | LEU | GLU | ALA | LEU | LYS | ||||
| 7 | GLU | HIS | CYS | GLU | VAL | ILE | LEU | SER | THR | ASP | ||||
| 8 | SER | GLN | TYR | VAL | ARG | GLN | GLY | ILE | THR | GLN | ||||
| 9 | TRP | ILE | HIS | ASN | TRP | LYS | LYS | ASN | GLY | TRP | ||||
| 10 | LYS | THR | ALA | ASP | LYS | LYS | PRO | VAL | LYS | ASN | ||||
| 11 | VAL | ASP | LEU | TRP | GLN | ARG | LEU | ASP | ALA | ALA | ||||
| 12 | LEU | GLY | GLN | HIS | GLN | ILE | LYS | TRP | GLU | TRP | ||||
| 13 | VAL | LYS | GLY | HIS | ALA | GLY | HIS | PRO | GLU | ASN | ||||
| 14 | GLU | ARG | CYS | ASP | GLU | LEU | ALA | ARG | ALA | ALA | ||||
| 15 | ALA | MET | ASN | PRO | THR | LEU | GLU | ASP | THR | GLY | ||||
| 16 | TYR | GLN | VAL | GLU | VAL |
Samples:
sample_1: R88N E. coli Ribonuclease HI, [U-100% 13C; U-100% 15N], 325 uM; deuterated sodium acetate 50 mM; sodium chloride 50 mM; sodium azide 0.02 % (w/v); DSS 3 mM
sample_conditions_1: ionic strength: 0.050 M; pH: 5.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts