BMRB Entry 51164

Name: Assignment of tRNAIle from Escherichia coli
Published: 2022-01-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51164

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Assignment of tRNAIle from Escherichia coli PubMed: 35275364

Deposition date: 2021-11-01 Original release date: 2022-01-23

Authors: de Jesus, Vanessa; Biedenbander, Thomas; Vogele, Jennifer; Wohnert, Jens; Furtig, Boris

Citation: de Jesus, Vanessa; Biedenbander, Thomas; Vogele, Jennifer; Wohnert, Jens; Furtig, Boris. "NMR assignment of non-modified tRNA Ile from Escherichia coli" Biomol. NMR Assign. 16, 165-170 (2022).

Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
entity_1: AGGCUUGUAGCUCAGGUGGU UAGAGCGCACCCCUGAUAAG GGUGAGGUCGGUGGUUCAAG UCCACUCAGGCCUACCA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	11
15N chemical shifts	74
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tRNAIle	1

Entities:

Entity 1, tRNAIle 77 residues - Formula weight is not available

1

A

G

C

U

G

U

A

G

2

C

U

C

A

G

U

G

U

3

U

A

G

A

G

C

G

C

A

C

4

C

U

G

A

U

A

G

5

G

U

G

A

G

U

C

G

6

G

U

G

U

C

A

G

7

U

C

A

C

U

C

A

G

8

C

U

A

C

A

Samples:

sample_1: tRNAIle, [U-100% 13C; U-100% 15N], 800 mM; D2O, [U-2H], 7%; DSS 250 mM; potassium chloride 200 mM; potassium phosphate 25 mM; magnesium chloride 5 mM

sample_conditions_1: ionic strength: 230 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

sample_conditions_3: ionic strength: 230 mM; pH: 6.2; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H,15N HNN-COSY	sample_1	isotropic	sample_conditions_1
2D 1H,15N BEST-TROSY	sample_1	isotropic	sample_conditions_1
2D 1H,1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H,1H NOESY	sample_1	isotropic	sample_conditions_3
2D 1H,15N CPMG NOESY	sample_1	isotropic	sample_conditions_1
2D 1H,15N 2J SOFAST HMQC	sample_1	isotropic	sample_conditions_1
13C-detected 2D (H)CN-HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2 only	sample_1	isotropic	sample_conditions_1
2D 1H,1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY v1.47 - chemical shift assignment

TOPSPIN v3.5 - processing

NMR spectrometers:

Bruker AVANCE IIIHD 600 MHz
Bruker AVANCE IIIHD 700 MHz
Bruker AVANCE III 800 MHz
Bruker AVANCE IIIHD 800 MHz
Bruker AVANCE NEO 900 MHz
Bruker AVANCE NEO 950 MHz