BMRB Entry 51170
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51170
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Title: 1H, 13C, 15N assignment of cyclosporin L in chloroform PubMed: 32307084
Deposition date: 2021-11-08 Original release date: 2021-11-12
Authors: Efimov, Sergey; Kobchikova, Polina; Klochkov, Vladimir
Citation: Efimov, Sergey; Dubinin, Mikhail; Kobchikova, Polina; Zgadzay, Yuriy; Khodov, Ilya; Belosludtsev, Konstantin; Klochkov, Vladimir. "Comparison of cyclosporin variants B-E based on their structural properties and activity in mitochondrial membranes" Biochem. Biophys. Res. Commun. 526, 1054-1060 (2020).
Assembly members:
entity_1, polymer, 11 residues, 1188.6 Da.
Natural source: Common Name: Tolypocladium inflatum Taxonomy ID: 29910 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Tolypocladium inflatum
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
entity_1: XXGLVLAXXXX
- assigned_chemical_shifts
- coupling_constants
Data type | Count |
13C chemical shifts | 61 |
15N chemical shifts | 8 |
1H chemical shifts | 90 |
coupling constants | 15 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CsL peptide | 1 |
Entities:
Entity 1, CsL peptide 11 residues - 1188.6 Da.
1 | BMT | ABU | SAR | MLE | VAL | MLE | ALA | DAL | MLE | MLE | ||||
2 | MVA |
Samples:
sample_1: CsL 1.75 mM
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1D 1H | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2 - collection
NMRPipe - processing
SPARKY - data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts