BMRB Entry 51172
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51172
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Title: Backbone NMR assignments of the chicken TRPV4 intrinsically disordered N-terminus PubMed: 35451798
Deposition date: 2021-11-09 Original release date: 2022-05-05
Authors: Goretzki, Benedikt; Tebbe, Frederike; Mitrovic, Sarah-Ana; Hellmich, Ute
Citation: Goretzki, Benedikt; Tebbe, Frederike; Mitrovic, Sarah-Ana; Hellmich, Ute. "Backbone NMR assignments of the extensive human and chicken TRPV4 N-terminal intrinsically disordered regions as important players in ion channel regulation" Biomol. NMR Assignments 16, 205-212 (2022).
Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
entity_1: ADPEDPRDAGDVLGDDSFPL
SSLANLFEVEDTPSPAEPSR
GPPGAVDGKQNLRMKFHGAF
RKGPPKPMELLESTIYESSV
VPAPKKAPMDSLFDYGTYRQ
HPSENKRWRRRVVEKPVAGT
KGPAPNPPPVLKV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 377 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 451 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IDR | 1 |
Entities:
Entity 1, IDR 133 residues - Formula weight is not available
Residues 2-134 of the chicken TRPV4 channel (UniProtKB - A0A1D5PXA5)
| 1 | ALA | ASP | PRO | GLU | ASP | PRO | ARG | ASP | ALA | GLY | ||||
| 2 | ASP | VAL | LEU | GLY | ASP | ASP | SER | PHE | PRO | LEU | ||||
| 3 | SER | SER | LEU | ALA | ASN | LEU | PHE | GLU | VAL | GLU | ||||
| 4 | ASP | THR | PRO | SER | PRO | ALA | GLU | PRO | SER | ARG | ||||
| 5 | GLY | PRO | PRO | GLY | ALA | VAL | ASP | GLY | LYS | GLN | ||||
| 6 | ASN | LEU | ARG | MET | LYS | PHE | HIS | GLY | ALA | PHE | ||||
| 7 | ARG | LYS | GLY | PRO | PRO | LYS | PRO | MET | GLU | LEU | ||||
| 8 | LEU | GLU | SER | THR | ILE | TYR | GLU | SER | SER | VAL | ||||
| 9 | VAL | PRO | ALA | PRO | LYS | LYS | ALA | PRO | MET | ASP | ||||
| 10 | SER | LEU | PHE | ASP | TYR | GLY | THR | TYR | ARG | GLN | ||||
| 11 | HIS | PRO | SER | GLU | ASN | LYS | ARG | TRP | ARG | ARG | ||||
| 12 | ARG | VAL | VAL | GLU | LYS | PRO | VAL | ALA | GLY | THR | ||||
| 13 | LYS | GLY | PRO | ALA | PRO | ASN | PRO | PRO | PRO | VAL | ||||
| 14 | LEU | LYS | VAL |
Samples:
sample_1: TRPV4-IDR, [U-13C; U-15N], 200 uM; DSS 100 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM
sample_conditions_1: ionic strength: 150 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.9.1.4 - chemical shift assignment
TOPSPIN v4.1 - processing
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts