BMRB Entry 51187

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PDB ID: 8eod
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51187
MolProbity Validation Chart

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Title:
Backbone and sidechain NMR assignments of the J-domain of co-chaperone Sis1 in complex with EEVD peptide of HSP70
Deposition date:
2021-11-23
Original release date:
2022-11-23
Authors:
Matos, Carolina; Pinheiro, Glaucia; Ramos, Carlos; Almeida, Fabio
Citation:

Citation: Matos, Carolina; Pinheiro, Glaucia; Ramos, Carlos; Almeida, Fabio. "Backbone and sidechain NMR assignments of residues 1-81 from yeast Sis1 in complex with an Hsp70 C-terminal EEVD peptide"  Biomol. NMR Assign. 17, 239-242 (2023).
PubMed: 37589820

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
entity_2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMTSVKETKLYDLLGVSPSA NEQELKKGYRKAALKYHPDK PTGDTEKFKEISEAFEILND PQKREIYDQYGLEAARSGGP SFGP
entity_2: GPTIEEVD

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts77
1H chemical shifts548

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1J-domain of Sis11
2HSP70 C-terminal tail octapeptide2

Entities:

Entity 1, J-domain of Sis1 84 residues - Formula weight is not available

1   GLYMETTHRSERVALLYSGLUTHRLYSLEU
2   TYRASPLEULEUGLYVALSERPROSERALA
3   ASNGLUGLNGLULEULYSLYSGLYTYRARG
4   LYSALAALALEULYSTYRHISPROASPLYS
5   PROTHRGLYASPTHRGLULYSPHELYSGLU
6   ILESERGLUALAPHEGLUILELEUASNASP
7   PROGLNLYSARGGLUILETYRASPGLNTYR
8   GLYLEUGLUALAALAARGSERGLYGLYPRO
9   SERPHEGLYPRO

Entity 2, HSP70 C-terminal tail octapeptide 8 residues - Formula weight is not available

1   GLYPROTHRILEGLUGLUVALASP

Related Database Links:

UNIPROT P11142 P11142
AlphaFold Q9H3R6 Q9H3R6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks