BMRB Entry 51188

Name: Backbone and ILV side chain assignment of the catalytic domain of human deubiquitinating enzyme USP7
Published: 2021-12-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51188

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone and ILV side chain assignment of the catalytic domain of human deubiquitinating enzyme USP7 PubMed: 35536398

Deposition date: 2021-11-23 Original release date: 2021-12-19

Authors: Korzhnev, Dmitry; Valles, Gabrielle; Pozhidaeva, Alexandra; Bezsonova, Irina

Citation: Valles, Gabrielle; Pozhidaeva, Alexandra; Korzhnev, Dmitry; Bezsonova, Irina. "Backbone and ILV side-chain NMR resonance assignments of the catalytic domain of human deubiquitinating enzyme USP7" Biomol. NMR Assign. 16, 197-203 (2022).

Assembly members:
entity_1, polymer, 356 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a-LIC

Entity Sequences (FASTA):
entity_1: GSSKKHTGYVGLKNQGATCY MNSLLQTLFFTNQLRKAVYM MPTEGDDSSKSVPLALQRVF YELQHSDKPVGTKKLTKSFG WETLDSFMQHDVQELCRVLL DNVENKMKGTCVEGTIPKLF RGKMVSYIQCKEVDYRSDRR EDYYDIQLSIKGKKNIFESF VDYVAVEQLDGDNKYDAGEH GLQEAEKGVKFLTLPPVLHL QLMRFMYDPQTDQNIKINDR FEFPEQLPLDEFLQKTDPKD PANYILHAVLVHSGDNHGGH YVVYLNPKGDGKWCKFDDDV VSRCTKEEAIEHNYGGHDDD LSVRHCTNAYMLVYIRESKL SEVLQAVTDHDIPQQLVERL QEEKRIEAQKRKERQE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	1135
15N chemical shifts	315
1H chemical shifts	690

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	USP7	1

Entities:

Entity 1, USP7 356 residues - Formula weight is not available

1

GLY

SER

LYS

HIS

THR

GLY

TYR

VAL

2

GLY

LEU

LYS

ASN

GLN

GLY

ALA

THR

CYS

TYR

3

MET

ASN

SER

LEU

GLN

THR

LEU

PHE

4

THR

ASN

GLN

LEU

ARG

LYS

ALA

VAL

TYR

MET

5

MET

PRO

THR

GLU

GLY

ASP

SER

LYS

6

SER

VAL

PRO

LEU

ALA

LEU

GLN

ARG

VAL

PHE

7

TYR

GLU

LEU

GLN

HIS

SER

ASP

LYS

PRO

VAL

8

GLY

THR

LYS

LEU

THR

LYS

SER

PHE

GLY

9

TRP

GLU

THR

LEU

ASP

SER

PHE

MET

GLN

HIS

10

ASP

VAL

GLN

GLU

LEU

CYS

ARG

VAL

LEU

11

ASP

ASN

VAL

GLU

ASN

LYS

MET

LYS

GLY

THR

12

CYS

VAL

GLU

GLY

THR

ILE

PRO

LYS

LEU

PHE

13

ARG

GLY

LYS

MET

VAL

SER

TYR

ILE

GLN

CYS

14

LYS

GLU

VAL

ASP

TYR

ARG

SER

ASP

ARG

15

GLU

ASP

TYR

ASP

ILE

GLN

LEU

SER

ILE

16

LYS

GLY

LYS

ASN

ILE

PHE

GLU

SER

PHE

17

VAL

ASP

TYR

VAL

ALA

VAL

GLU

GLN

LEU

ASP

18

GLY

ASP

ASN

LYS

TYR

ASP

ALA

GLY

GLU

HIS

19

GLY

LEU

GLN

GLU

ALA

GLU

LYS

GLY

VAL

LYS

20

PHE

LEU

THR

LEU

PRO

VAL

LEU

HIS

LEU

21

GLN

LEU

MET

ARG

PHE

MET

TYR

ASP

PRO

GLN

22

THR

ASP

GLN

ASN

ILE

LYS

ILE

ASN

ASP

ARG

23

PHE

GLU

PHE

PRO

GLU

GLN

LEU

PRO

LEU

ASP

24

GLU

PHE

LEU

GLN

LYS

THR

ASP

PRO

LYS

ASP

25

PRO

ALA

ASN

TYR

ILE

LEU

HIS

ALA

VAL

LEU

26

VAL

HIS

SER

GLY

ASP

ASN

HIS

GLY

HIS

27

TYR

VAL

TYR

LEU

ASN

PRO

LYS

GLY

ASP

28

GLY

LYS

TRP

CYS

LYS

PHE

ASP

VAL

29

VAL

SER

ARG

CYS

THR

LYS

GLU

ALA

ILE

30

GLU

HIS

ASN

TYR

GLY

HIS

ASP

31

LEU

SER

VAL

ARG

HIS

CYS

THR

ASN

ALA

TYR

32

MET

LEU

VAL

TYR

ILE

ARG

GLU

SER

LYS

LEU

33

SER

GLU

VAL

LEU

GLN

ALA

VAL

THR

ASP

HIS

34

ASP

ILE

PRO

GLN

LEU

VAL

GLU

ARG

LEU

35

GLN

GLU

LYS

ARG

ILE

GLU

ALA

GLN

LYS

36

ARG

LYS

GLU

ARG

GLN

GLU

Samples:

sample_1: USP7, [U-15N; U-2H; U-13C], 1 mM; TRIS HCL 20 mM; NaCl 100 mM; DTT 2 mM

sample_conditions_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C TROSY-HMQC	sample_1	isotropic	sample_conditions_1
HMCMCGCBCACO	sample_1	isotropic	sample_conditions_1
HMCMCGCBCA	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Agilent VXRS 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts