BMRB Entry 51197

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51197

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Title:
1H, 15N Backbone Assignments of Rec3 from GeoCas9
Deposition date:
2021-11-29
Original release date:
2024-07-22
Authors:
Belato, Helen
Citation:

Citation: Belato, Helen; Knight, Alexa; D'Ordine, Alexandra; Fan, Zhiqiang; Luo, Jinping; Jogl, Gerwald; Lisi, George. "Atomistic Tuning of the GeoCas9 Recognition Lobe Modulates Allosteric Motions and Guide RNA Interactions"  bioRxiv ., .-. (2024).
PubMed: 38746279

Assembly members:

Assembly members:
entity_1, polymer, 212 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KEKRAPKATYTFQSFIAWEH INKLRLISPSGARGLTDEER RLLYEQAFQKNKITYHDIRT LLHLPDDTYFKGIVYDRGES RKQNENIRFLELDAYHQIRK AVDKVYGKGKSSSFLPIDFD TFGYALTLFKDDADIHSYLR NEYEQNGKRMPNLANKVYDN ELIEELLNLSFTKFGHLSLK ALRSILPYMEQGEVYSSACE RAGYTFTGPKKK

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Rec31

Entities:

Entity 1, Rec3 212 residues - Formula weight is not available

1   LYSGLULYSARGALAPROLYSALATHRTYR
2   THRPHEGLNSERPHEILEALATRPGLUHIS
3   ILEASNLYSLEUARGLEUILESERPROSER
4   GLYALAARGGLYLEUTHRASPGLUGLUARG
5   ARGLEULEUTYRGLUGLNALAPHEGLNLYS
6   ASNLYSILETHRTYRHISASPILEARGTHR
7   LEULEUHISLEUPROASPASPTHRTYRPHE
8   LYSGLYILEVALTYRASPARGGLYGLUSER
9   ARGLYSGLNASNGLUASNILEARGPHELEU
10   GLULEUASPALATYRHISGLNILEARGLYS
11   ALAVALASPLYSVALTYRGLYLYSGLYLYS
12   SERSERSERPHELEUPROILEASPPHEASP
13   THRPHEGLYTYRALALEUTHRLEUPHELYS
14   ASPASPALAASPILEHISSERTYRLEUARG
15   ASNGLUTYRGLUGLNASNGLYLYSARGMET
16   PROASNLEUALAASNLYSVALTYRASPASN
17   GLULEUILEGLUGLULEULEUASNLEUSER
18   PHETHRLYSPHEGLYHISLEUSERLEULYS
19   ALALEUARGSERILELEUPROTYRMETGLU
20   GLNGLYGLUVALTYRSERSERALACYSGLU
21   ARGALAGLYTYRTHRPHETHRGLYPROLYS
22   LYSLYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks