BMRB Entry 51198

Name: Tandem WW domains in WWOX: the multifaceted role of WW2
Published: 2022-10-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51198

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Tandem WW domains in WWOX: the multifaceted role of WW2 PubMed: 35716775

Deposition date: 2021-11-30 Original release date: 2022-10-12

Authors: Adamsky-Keinan, Keren; Rotem-Bamberger, Shahar; Shalev, Deborah; Schueler-Furman, Ora; Chill, Jordan

Citation: Rotem-Bamberger, Shahar; Fahoum, Jamal; Keinan-Adamsky, Keren; Tsaban, Tomer; Avraham, Orly; Shalev, Deborah; Chill, Jordan; Schueler-Furman, Ora. "Structural insights into the role of the WW2 domain on tandem WW-PPxY motif interactions of oxidoreductase WWOX" J. Biol. Chem. 298, 102145-102145 (2022).

Assembly members:
entity_1, polymer, 80 residues, 9379.28 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM 30

Entity Sequences (FASTA):
entity_1: GAMGDELPPGWEERTTKDGW VYYANHTEEKTQWEHPKTGK RKRVAGDLPYGWEQETDENG QVFFVDHINKRTTYLDPRLA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	61
1H chemical shifts	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WW1WW2	1

Entities:

Entity 1, WW1WW2 80 residues - 9379.28 Da.

1	GLY	ALA	MET	GLY	ASP	GLU	LEU	PRO	PRO	GLY
2	TRP	GLU	GLU	ARG	THR	THR	LYS	ASP	GLY	TRP
3	VAL	TYR	TYR	ALA	ASN	HIS	THR	GLU	GLU	LYS
4	THR	GLN	TRP	GLU	HIS	PRO	LYS	THR	GLY	LYS
5	ARG	LYS	ARG	VAL	ALA	GLY	ASP	LEU	PRO	TYR
6	GLY	TRP	GLU	GLN	GLU	THR	ASP	GLU	ASN	GLY
7	GLN	VAL	PHE	PHE	VAL	ASP	HIS	ILE	ASN	LYS
8	ARG	THR	THR	TYR	LEU	ASP	PRO	ARG	LEU	ALA

Samples:

sample_1: H2O 93 ± 1 %; D2O, [U-99% 2H], 7 ± 1 %; sodium phosphate 20 ± 1 mM; sodium chloride 100 ± 1 mM; AEBSF protease inhibitor 0.1 ± 0.01 %; WW1WW2, [U-99% 13C; U-99% 15N], 0.2 ± 0.005 mM

sample_conditions_1: ionic strength: 0.032 M; pH: 6.8; pressure: 1 atm; temperature: 286 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6 - chemical shift assignment, collection

NMR spectrometers:

Bruker DRX 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	GLY	ALA	MET	GLY	ASP	GLU	LEU	PRO	PRO	GLY
2	TRP	GLU	GLU	ARG	THR	THR	LYS	ASP	GLY	TRP
3	VAL	TYR	TYR	ALA	ASN	HIS	THR	GLU	GLU	LYS
4	THR	GLN	TRP	GLU	HIS	PRO	LYS	THR	GLY	LYS
5	ARG	LYS	ARG	VAL	ALA	GLY	ASP	LEU	PRO	TYR
6	GLY	TRP	GLU	GLN	GLU	THR	ASP	GLU	ASN	GLY
7	GLN	VAL	PHE	PHE	VAL	ASP	HIS	ILE	ASN	LYS
8	ARG	THR	THR	TYR	LEU	ASP	PRO	ARG	LEU	ALA

1	GLY	ALA	MET	GLY	ASP	GLU	LEU	PRO	PRO	GLY
2	TRP	GLU	GLU	ARG	THR	THR	LYS	ASP	GLY	TRP
3	VAL	TYR	TYR	ALA	ASN	HIS	THR	GLU	GLU	LYS
4	THR	GLN	TRP	GLU	HIS	PRO	LYS	THR	GLY	LYS
5	ARG	LYS	ARG	VAL	ALA	GLY	ASP	LEU	PRO	TYR
6	GLY	TRP	GLU	GLN	GLU	THR	ASP	GLU	ASN	GLY
7	GLN	VAL	PHE	PHE	VAL	ASP	HIS	ILE	ASN	LYS
8	ARG	THR	THR	TYR	LEU	ASP	PRO	ARG	LEU	ALA

1	GLY	ALA	MET	GLY	ASP	GLU	LEU	PRO	PRO	GLY
2	TRP	GLU	GLU	ARG	THR	THR	LYS	ASP	GLY	TRP
3	VAL	TYR	TYR	ALA	ASN	HIS	THR	GLU	GLU	LYS
4	THR	GLN	TRP	GLU	HIS	PRO	LYS	THR	GLY	LYS
5	ARG	LYS	ARG	VAL	ALA	GLY	ASP	LEU	PRO	TYR
6	GLY	TRP	GLU	GLN	GLU	THR	ASP	GLU	ASN	GLY
7	GLN	VAL	PHE	PHE	VAL	ASP	HIS	ILE	ASN	LYS
8	ARG	THR	THR	TYR	LEU	ASP	PRO	ARG	LEU	ALA