BMRB Entry 51201

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51201

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Title:
1H-15N HSQC spectrum of cGMP-bound human PKG CBD-B
Deposition date:
2021-11-30
Original release date:
2022-03-04
Authors:
Byun, Jung; VanSchouwen, Bryan; Huang, Jinfeng; Baryar, Ubaidullah; Melacini, Giuseppe
Citation:

Citation: Byun, Jung; VanSchouwen, Bryan; Huang, Jinfeng; Baryar, Ubaidullah; Melacini, Giuseppe. "Divergent allostery reveals critical differences between structurally homologous regulatory domains of Plasmodium falciparum and human protein kinase G"  J. Biol. Chem. 298, 101691-101691 (2022).
PubMed: 35143840

Assembly members:

Assembly members:
entity_1, polymer, 153 residues, Formula weight is not available
entity_PCG, non-polymer, 345.205 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQTEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSTGLIKHTEYMEFLKSVPT FQSLPEEILSKLADVLEETH YENGEYIIRQGARGDTFFII SKGTVNVTREDSPSEDPVFL RTLGKGDWFGEKALQGEDVR TANVIAAEAVTCLVIDRDSF KHLIGGLDDVSNKAYEDAEA KAKYEAEAAFFAN

Data sets:
Data typeCount
15N chemical shifts125
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Human protein kinase G isoform Ib CBD-B domain1
2Cyclic guanosine monophosphate2

Entities:

Entity 1, Human protein kinase G isoform Ib CBD-B domain 153 residues - Formula weight is not available

Residues 1 and 2 represent part of a non-native N-terminal affinity tag. The remaining residues represent residues 219-369 of the CBD-B domain of human protein kinase G isoform Ib.

1   GLYSERTHRGLYLEUILELYSHISTHRGLU
2   TYRMETGLUPHELEULYSSERVALPROTHR
3   PHEGLNSERLEUPROGLUGLUILELEUSER
4   LYSLEUALAASPVALLEUGLUGLUTHRHIS
5   TYRGLUASNGLYGLUTYRILEILEARGGLN
6   GLYALAARGGLYASPTHRPHEPHEILEILE
7   SERLYSGLYTHRVALASNVALTHRARGGLU
8   ASPSERPROSERGLUASPPROVALPHELEU
9   ARGTHRLEUGLYLYSGLYASPTRPPHEGLY
10   GLULYSALALEUGLNGLYGLUASPVALARG
11   THRALAASNVALILEALAALAGLUALAVAL
12   THRCYSLEUVALILEASPARGASPSERPHE
13   LYSHISLEUILEGLYGLYLEUASPASPVAL
14   SERASNLYSALATYRGLUASPALAGLUALA
15   LYSALALYSTYRGLUALAGLUALAALAPHE
16   PHEALAASN

Entity 2, Cyclic guanosine monophosphate - C10 H12 N5 O7 P - 345.205 Da.

1   PCG

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks