BMRB Entry 51210

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51210

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Title:
Backbone and sidechain 1H, 15N and 13C resonance assignments of the RNA bound tandem zinc finger domain of the tristetraprolin family member from Selaginella moellendorffii
Deposition date:
2021-12-02
Original release date:
2022-12-02
Authors:
Hicks, Stephanie; Venters, Ronald; Blackshear, Perry
Citation:

Citation: Hicks, Stephanie; Venters, Ronald; Blackshear, Perry. "Backbone and sidechain 1H, 15N and 13C resonance assignments of the free and RNA-bound tandem zinc finger domain of the tristetraprolin family member from Selaginella moellendorffii"  Biomol. NMR Assign. 16, 153-158 (2022).
PubMed: 35279790

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, 7784.89 Da.
entity_ZN, non-polymer, 65.409 Da.
entity_3, polymer, 9 residues, 2739.6 Da.

Natural source:

Natural source:   Common Name: Selaginella moellendorffii   Taxonomy ID: 88036   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Selaginella moellendorffii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28/30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NALYKTELCRSWEETGSCRY GNKCQFAHGKEDLRPVNRHP KYKTEVCRTFSAAGTCPYGK RCRFIHATP
entity_3: UUAUUUAUU

Data sets:
Data typeCount
13C chemical shifts287
15N chemical shifts62
1H chemical shifts422

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tandem Zinc Finger1
2Zn12
3Zn22
49mer RNA3

Entities:

Entity 1, Tandem Zinc Finger 69 residues - 7784.89 Da.

This tandem zinc finger domain represents residues 10-76 from the full length protein; The first two residues in the assigned chemical shifts list (ASN and ALA) are not part of the S. moellendorfii TTP TZF sequence but are there because of the TEV cleavage site.

1   ASNALALEUTYRLYSTHRGLULEUCYSARG
2   SERTRPGLUGLUTHRGLYSERCYSARGTYR
3   GLYASNLYSCYSGLNPHEALAHISGLYLYS
4   GLUASPLEUARGPROVALASNARGHISPRO
5   LYSTYRLYSTHRGLUVALCYSARGTHRPHE
6   SERALAALAGLYTHRCYSPROTYRGLYLYS
7   ARGCYSARGPHEILEHISALATHRPRO

Entity 2, Zn1 - Zn - 65.409 Da.

1   ZN

Entity 3, 9mer RNA 9 residues - 2739.6 Da.

1   UUAUUUAUU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks