BMRB Entry 51211

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51211

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Title:
13C/15N/1H chemical shift assignments for Human Cytomegalovirus Protease delta221
Deposition date:
2021-12-03
Original release date:
2022-04-07
Authors:
Lee, Gregory; Craik, Charles
Citation:

Citation: Hulce, Kaitlin; Jaishankar, Priyadarshini; Lee, Gregory; Bohn, Markus-Frederik; Connelly, Emily; Wucherer, Kristin; Volk, Regan; Chuo, Shih-Wei; Arkin, Michelle; Renslo, Adam; Craik, Charles. "Inhibiting a dynamic viral protease by targeting a non-catalytic cysteine"  Cell Chem. Biol. 29, 785-798 (2022).
PubMed: 35364007

Assembly members:

Assembly members:
entity_1, polymer, 228 residues, 24033.9 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHMTMDEQQSQAVAP VYVGGFLARYDQSPDEAELL LPRDVVEHWLHAQGQGQPSL SVALPLNINHDDTAVVGHVA AMQSVRDGLFCLGCVTSPRF LEIVRRASEKSELVSRGPVS PLQPDKVVEFLSGSYAGLSL SSRRCDDVEVATSLSGSETT PFKHVALCSVGRRRGTLAVY GRDPEWVTQRFPDLTAADRD GLRAQWQRCGSTAVDVSGDP FRSDSYGL

Data sets:
Data typeCount
13C chemical shifts695
15N chemical shifts203
1H chemical shifts592

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1HCMV Pr delta2211

Entities:

Entity 1, HCMV Pr delta221 228 residues - 24033.9 Da.

Contains a non-native N-terminal Met+His6-tag (residues -7 to -1). Residue numbering begins at Met1. This is a C-terminal truncated construct that ends with L221.

1   METHISHISHISHISHISHISMETTHRMET
2   ASPGLUGLNGLNSERGLNALAVALALAPRO
3   VALTYRVALGLYGLYPHELEUALAARGTYR
4   ASPGLNSERPROASPGLUALAGLULEULEU
5   LEUPROARGASPVALVALGLUHISTRPLEU
6   HISALAGLNGLYGLNGLYGLNPROSERLEU
7   SERVALALALEUPROLEUASNILEASNHIS
8   ASPASPTHRALAVALVALGLYHISVALALA
9   ALAMETGLNSERVALARGASPGLYLEUPHE
10   CYSLEUGLYCYSVALTHRSERPROARGPHE
11   LEUGLUILEVALARGARGALASERGLULYS
12   SERGLULEUVALSERARGGLYPROVALSER
13   PROLEUGLNPROASPLYSVALVALGLUPHE
14   LEUSERGLYSERTYRALAGLYLEUSERLEU
15   SERSERARGARGCYSASPASPVALGLUVAL
16   ALATHRSERLEUSERGLYSERGLUTHRTHR
17   PROPHELYSHISVALALALEUCYSSERVAL
18   GLYARGARGARGGLYTHRLEUALAVALTYR
19   GLYARGASPPROGLUTRPVALTHRGLNARG
20   PHEPROASPLEUTHRALAALAASPARGASP
21   GLYLEUARGALAGLNTRPGLNARGCYSGLY
22   SERTHRALAVALASPVALSERGLYASPPRO
23   PHEARGSERASPSERTYRGLYLEU

Related Database Links:

NCBI 2554902

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks