BMRB Entry 51229

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51229

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Title:
The N-terminal domain of RNAse Y is organized as a coiled-coil domain, which displays two different conformations, one of them binding enolase.
Deposition date:
2021-12-14
Original release date:
2024-08-09
Authors:
Hardouin, Pierre; Morellet, Nelly; Assrir, Nadine; Bou Nader, Charles; van Heijenoort, Carine; Golinelli-Pimpaneau, Beatrice
Citation:

Citation: Morellet, Nelly; Hardouin, Pierre; Assrir, Nadine; van Heijenoort, Carine; Golinelli-Pimpaneau, Beatrice. "Structural Insights into the Dimeric Form of Bacillus subtilis RNase Y Using NMR and AlphaFold"  Biomolecules 12, 1798-1798 (2022).
PubMed: 36551226

Assembly members:

Assembly members:
entity_1, polymer, 176 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3) star

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MVRKTIAEAKIAGARGAAEQ ILEDAKRDAEALKKEALLEA KDEIHKLRIDAEQEVRERRN ELQKQENRLLQKEENLDRKH EGIDKREAMLEKKDHSLNER QQHIEEMESKVDEMIRMQQS ELERISSLTRDEAKQIILER VENELSHDIAIMTKETENRA KEEADKKAKNHHHHHH

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts151
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Nter-BsRNaseY, chain A1
2Nter-BsRNaseY, chain B1

Entities:

Entity 1, Nter-BsRNaseY, chain A 176 residues - Formula weight is not available

1   METVALARGLYSTHRILEALAGLUALALYS
2   ILEALAGLYALAARGGLYALAALAGLUGLN
3   ILELEUGLUASPALALYSARGASPALAGLU
4   ALALEULYSLYSGLUALALEULEUGLUALA
5   LYSASPGLUILEHISLYSLEUARGILEASP
6   ALAGLUGLNGLUVALARGGLUARGARGASN
7   GLULEUGLNLYSGLNGLUASNARGLEULEU
8   GLNLYSGLUGLUASNLEUASPARGLYSHIS
9   GLUGLYILEASPLYSARGGLUALAMETLEU
10   GLULYSLYSASPHISSERLEUASNGLUARG
11   GLNGLNHISILEGLUGLUMETGLUSERLYS
12   VALASPGLUMETILEARGMETGLNGLNSER
13   GLULEUGLUARGILESERSERLEUTHRARG
14   ASPGLUALALYSGLNILEILELEUGLUARG
15   VALGLUASNGLULEUSERHISASPILEALA
16   ILEMETTHRLYSGLUTHRGLUASNARGALA
17   LYSGLUGLUALAASPLYSLYSALALYSASN
18   HISHISHISHISHISHIS

Related Database Links:

NCBI WP_003221010.1

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