BMRB Entry 51237

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51237

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Title:
Resonance assignments of a phsophomimetic of the C-terminal domain of the P protein of the Nishigahara strain of rabies virus
Deposition date:
2021-12-19
Original release date:
2022-03-29
Authors:
Zhan, Jingyu; Gooley, Paul
Citation:

Citation: Zhan, Jingyu; Watts, Ericka; Brice, Aaron; Metcalfe, Riley; Sethi, Ashish; Yan, Fei; Rozario, Ashley; Bell, Toby; Griffin, Michael; Moseley, Gregory; Gooley, Paul. "Molecular basis of functional effects of protein kinase C phosphorylation of rabies virus P protein"  J. Virol. 96, e0011122-e0011122 (2022).
PubMed: 35404083

Assembly members:

Assembly members:
entity_1, polymer, 115 residues, 13042.86 Da.

Natural source:

Natural source:   Common Name: Rabies lyssavirus   Taxonomy ID: not available   Superkingdom: Viruses   Kingdom: not available   Genus/species: lyssavirus Rabies lyssavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMWSATNEEDDLSVEAEIA HQIAESFEKKYKFPSRSSGI FLYNFEQLKMNLDDIVKEAK NVPGVTRLAHDGSKLPLRCV LGWVALANSKKFQLLVEANK LNKIMQDDLNRYASS

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts122
1H chemical shifts134

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain1

Entities:

Entity 1, C-terminal domain 115 residues - 13042.86 Da.

The first 3 residues GHM represent a non-native part introduced from cloning.

1   GLYHISMETTRPSERALATHRASNGLUGLU
2   ASPASPLEUSERVALGLUALAGLUILEALA
3   HISGLNILEALAGLUSERPHEGLULYSLYS
4   TYRLYSPHEPROSERARGSERSERGLYILE
5   PHELEUTYRASNPHEGLUGLNLEULYSMET
6   ASNLEUASPASPILEVALLYSGLUALALYS
7   ASNVALPROGLYVALTHRARGLEUALAHIS
8   ASPGLYSERLYSLEUPROLEUARGCYSVAL
9   LEUGLYTRPVALALALEUALAASNSERLYS
10   LYSPHEGLNLEULEUVALGLUALAASNLYS
11   LEUASNLYSILEMETGLNASPASPLEUASN
12   ARGTYRALASERSER

Related Database Links:

UniProt Q9IPJ8
AlphaFold Q75T11

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks