BMRB Entry 51253
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51253
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution NMR backbone chemical shift assignment for the La Module of human La-related protein 7 PubMed: 35320752
Deposition date: 2021-12-22 Original release date: 2022-03-28
Authors: Yang, Yuan; Hadjian, Tanya; Feigon, Juli
Citation: Yang, Yuan; Liu, Shiheng; Egloff, Sylvain; Eichhorn, Catherine; Hadjian, Tanya; Zhen, James; Kiss, Tamas; Zhou, Z. Hong; Feigon, Juli. "Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNP" Mol. Cell 82, 1724-1736 (2022).
Assembly members:
entity_1, polymer, 202 residues, 23424 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet-1
Entity Sequences (FASTA):
entity_1: GEVEKKKRSRVKQVLADIAK
QVDFWFGDANLHKDRFLREQ
IEKSRDGYVDISLLVSFNKM
KKLTTDGKLIARALRSSAVV
ELDLEGTRIRRKKPLGERPK
DEDERTVYVELLPKNVNHSW
IERVFGKCGNVVYISIPHYK
STGDPKGFAFVEFETKEQAA
KAIEFLNNPPEEAPRKERHK
MGEEVIPLRVLSKSEWMDLK
KE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 499 |
| 15N chemical shifts | 182 |
| 1H chemical shifts | 182 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Larp7 La module | 1 |
Entities:
Entity 1, Larp7 La module 202 residues - 23424 Da.
First Glycine (residue 21) is a non-native residue as a result of TEV-protease cleavage site. Loop truncation between (including) native residues 197 and 364.
| 1 | GLY | GLU | VAL | GLU | LYS | LYS | LYS | ARG | SER | ARG | ||||
| 2 | VAL | LYS | GLN | VAL | LEU | ALA | ASP | ILE | ALA | LYS | ||||
| 3 | GLN | VAL | ASP | PHE | TRP | PHE | GLY | ASP | ALA | ASN | ||||
| 4 | LEU | HIS | LYS | ASP | ARG | PHE | LEU | ARG | GLU | GLN | ||||
| 5 | ILE | GLU | LYS | SER | ARG | ASP | GLY | TYR | VAL | ASP | ||||
| 6 | ILE | SER | LEU | LEU | VAL | SER | PHE | ASN | LYS | MET | ||||
| 7 | LYS | LYS | LEU | THR | THR | ASP | GLY | LYS | LEU | ILE | ||||
| 8 | ALA | ARG | ALA | LEU | ARG | SER | SER | ALA | VAL | VAL | ||||
| 9 | GLU | LEU | ASP | LEU | GLU | GLY | THR | ARG | ILE | ARG | ||||
| 10 | ARG | LYS | LYS | PRO | LEU | GLY | GLU | ARG | PRO | LYS | ||||
| 11 | ASP | GLU | ASP | GLU | ARG | THR | VAL | TYR | VAL | GLU | ||||
| 12 | LEU | LEU | PRO | LYS | ASN | VAL | ASN | HIS | SER | TRP | ||||
| 13 | ILE | GLU | ARG | VAL | PHE | GLY | LYS | CYS | GLY | ASN | ||||
| 14 | VAL | VAL | TYR | ILE | SER | ILE | PRO | HIS | TYR | LYS | ||||
| 15 | SER | THR | GLY | ASP | PRO | LYS | GLY | PHE | ALA | PHE | ||||
| 16 | VAL | GLU | PHE | GLU | THR | LYS | GLU | GLN | ALA | ALA | ||||
| 17 | LYS | ALA | ILE | GLU | PHE | LEU | ASN | ASN | PRO | PRO | ||||
| 18 | GLU | GLU | ALA | PRO | ARG | LYS | GLU | ARG | HIS | LYS | ||||
| 19 | MET | GLY | GLU | GLU | VAL | ILE | PRO | LEU | ARG | VAL | ||||
| 20 | LEU | SER | LYS | SER | GLU | TRP | MET | ASP | LEU | LYS | ||||
| 21 | LYS | GLU |
Samples:
sample_1: Larp7 La module 22-390 with loop deletion, [U-13C; U-15N], 0.8 mM; sodium phosphate 20 mM; potassium chloride 50 mM; TCEP 1 mM; D2O 5%
sample_conditions_1: ionic strength: 0.07 M; pH: 6.05; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN vv4.0.7 - collection
TOPSPIN vv3.5pl7 - collection
NMRPipe vv2016.273.11.31 - processing
NMRFAM-SPARKY vv1.470 - chemical shift assignment
NMR spectrometers:
- Bruker Avance Neo 800 MHz
- Bruker Avance III HD 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts