BMRB Entry 51253

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51253

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Title:
Solution NMR backbone chemical shift assignment for the La Module of human La-related protein 7
Deposition date:
2021-12-22
Original release date:
2022-03-28
Authors:
Yang, Yuan; Hadjian, Tanya; Feigon, Juli
Citation:

Citation: Yang, Yuan; Liu, Shiheng; Egloff, Sylvain; Eichhorn, Catherine; Hadjian, Tanya; Zhen, James; Kiss, Tamas; Zhou, Z. Hong; Feigon, Juli. "Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNP"  Mol. Cell 82, 1724-1736 (2022).
PubMed: 35320752

Assembly members:

Assembly members:
entity_1, polymer, 202 residues, 23424 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GEVEKKKRSRVKQVLADIAK QVDFWFGDANLHKDRFLREQ IEKSRDGYVDISLLVSFNKM KKLTTDGKLIARALRSSAVV ELDLEGTRIRRKKPLGERPK DEDERTVYVELLPKNVNHSW IERVFGKCGNVVYISIPHYK STGDPKGFAFVEFETKEQAA KAIEFLNNPPEEAPRKERHK MGEEVIPLRVLSKSEWMDLK KE

Data sets:
Data typeCount
13C chemical shifts499
15N chemical shifts182
1H chemical shifts182

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Larp7 La module1

Entities:

Entity 1, Larp7 La module 202 residues - 23424 Da.

First Glycine (residue 21) is a non-native residue as a result of TEV-protease cleavage site. Loop truncation between (including) native residues 197 and 364.

1   GLYGLUVALGLULYSLYSLYSARGSERARG
2   VALLYSGLNVALLEUALAASPILEALALYS
3   GLNVALASPPHETRPPHEGLYASPALAASN
4   LEUHISLYSASPARGPHELEUARGGLUGLN
5   ILEGLULYSSERARGASPGLYTYRVALASP
6   ILESERLEULEUVALSERPHEASNLYSMET
7   LYSLYSLEUTHRTHRASPGLYLYSLEUILE
8   ALAARGALALEUARGSERSERALAVALVAL
9   GLULEUASPLEUGLUGLYTHRARGILEARG
10   ARGLYSLYSPROLEUGLYGLUARGPROLYS
11   ASPGLUASPGLUARGTHRVALTYRVALGLU
12   LEULEUPROLYSASNVALASNHISSERTRP
13   ILEGLUARGVALPHEGLYLYSCYSGLYASN
14   VALVALTYRILESERILEPROHISTYRLYS
15   SERTHRGLYASPPROLYSGLYPHEALAPHE
16   VALGLUPHEGLUTHRLYSGLUGLNALAALA
17   LYSALAILEGLUPHELEUASNASNPROPRO
18   GLUGLUALAPROARGLYSGLUARGHISLYS
19   METGLYGLUGLUVALILEPROLEUARGVAL
20   LEUSERLYSSERGLUTRPMETASPLEULYS
21   LYSGLU

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SPARKY: Backbone or all simulated peaks