BMRB Entry 51255
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51255
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Title: Larp1 PubMed: 35979957
Deposition date: 2022-01-01 Original release date: 2022-07-12
Authors: Nyandwi, Samuel; Sprules, Tara; Kozlov, Guennadi; Gehring, Kalle
Citation: Kozlov, Guennadi; Mattijssen, Sandy; Jiang, Jianning; Nyandwi, Samuel; Sprules, Tara; Iben, James; Coon, Steven; Gaidamakov, Sergei; Noronha, Anne; Wilds, Christopher; Maraia, Richard; Gehring, Kalle. "Structural basis of 3'-end poly(A) RNA recognition by LARP1" Nucleic Acids Res. 50, 9534-9547 (2022).
Assembly members:
entity_1, polymer, 99 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSQELLKDYIK
RQIEYYFSVDNLERDFFLRR
KMDADGFLPITLIASFHRVQ
ALTTDISLIFAALKDSKVVE
IVDEKVRRREEPEKWPLPP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 252 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Larp1 | 1 |
Entities:
Entity 1, Larp1 99 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | GLN | GLU | LEU | LEU | LYS | ASP | TYR | ILE | LYS | ||||
| 3 | ARG | GLN | ILE | GLU | TYR | TYR | PHE | SER | VAL | ASP | ||||
| 4 | ASN | LEU | GLU | ARG | ASP | PHE | PHE | LEU | ARG | ARG | ||||
| 5 | LYS | MET | ASP | ALA | ASP | GLY | PHE | LEU | PRO | ILE | ||||
| 6 | THR | LEU | ILE | ALA | SER | PHE | HIS | ARG | VAL | GLN | ||||
| 7 | ALA | LEU | THR | THR | ASP | ILE | SER | LEU | ILE | PHE | ||||
| 8 | ALA | ALA | LEU | LYS | ASP | SER | LYS | VAL | VAL | GLU | ||||
| 9 | ILE | VAL | ASP | GLU | LYS | VAL | ARG | ARG | ARG | GLU | ||||
| 10 | GLU | PRO | GLU | LYS | TRP | PRO | LEU | PRO | PRO |
Samples:
sample_1: LARP1, [U-100% 13C; U-100% 15N], 0.8 mM; sodium chloride 100 mM; MES 10 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5 pl5 - collection
NMRDraw - processing
NMRFAM-SPARKY - chemical shift assignment
NMRViewJ - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts