BMRB Entry 51256
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51256
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Inter-domain flexibility of human SRSF1 tandem RRMs allows flexibility in RNA binding PubMed: 36454680
Deposition date: 2022-01-03 Original release date: 2023-02-16
Authors: De Silva, Naiduwadura Ivon; Zhang, Jun
Citation: De Silva, Naiduwadura Ivon Upekala; Fargason, Talia; Zhang, Zihan; Wang, Ting; Zhang, Jun. "Inter-domain Flexibility of Human Ser/Arg-Rich Splicing Factor 1 Allows Variable Spacer Length in Cognate RNA's Bipartite Motifs" Biochemistry 61, 2922-2932 (2022).
Assembly members:
entity_1, polymer, 196 residues, Formula weight is not available
entity_2, polymer, 8 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSMT3
Entity Sequences (FASTA):
entity_1: MSGGGVIRGPAGNNDCRIYV
GNLPPDIRTKDIEDVFYKYG
AIRDIDLKNRRGGPPFAFVE
FEDPRDAEDAVYGRDGYDYD
GYRLRVEFPRSGRGTGRGGG
GGGGGGAPRGRYGPPSRRSE
NRVVVSGLPPSGSWQDLKDH
MREAGDVCYADVYRDGTGVV
EFVRKEDMTYAVRKLDNTKF
RSHEGETAYIRVKVDG
entity_2: UCAGAGGA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 485 |
| 15N chemical shifts | 164 |
| 1H chemical shifts | 164 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Protein | 1 |
| 2 | RNA | 2 |
Entities:
Entity 1, Protein 196 residues - Formula weight is not available
| 1 | MET | SER | GLY | GLY | GLY | VAL | ILE | ARG | GLY | PRO | ||||
| 2 | ALA | GLY | ASN | ASN | ASP | CYS | ARG | ILE | TYR | VAL | ||||
| 3 | GLY | ASN | LEU | PRO | PRO | ASP | ILE | ARG | THR | LYS | ||||
| 4 | ASP | ILE | GLU | ASP | VAL | PHE | TYR | LYS | TYR | GLY | ||||
| 5 | ALA | ILE | ARG | ASP | ILE | ASP | LEU | LYS | ASN | ARG | ||||
| 6 | ARG | GLY | GLY | PRO | PRO | PHE | ALA | PHE | VAL | GLU | ||||
| 7 | PHE | GLU | ASP | PRO | ARG | ASP | ALA | GLU | ASP | ALA | ||||
| 8 | VAL | TYR | GLY | ARG | ASP | GLY | TYR | ASP | TYR | ASP | ||||
| 9 | GLY | TYR | ARG | LEU | ARG | VAL | GLU | PHE | PRO | ARG | ||||
| 10 | SER | GLY | ARG | GLY | THR | GLY | ARG | GLY | GLY | GLY | ||||
| 11 | GLY | GLY | GLY | GLY | GLY | GLY | ALA | PRO | ARG | GLY | ||||
| 12 | ARG | TYR | GLY | PRO | PRO | SER | ARG | ARG | SER | GLU | ||||
| 13 | ASN | ARG | VAL | VAL | VAL | SER | GLY | LEU | PRO | PRO | ||||
| 14 | SER | GLY | SER | TRP | GLN | ASP | LEU | LYS | ASP | HIS | ||||
| 15 | MET | ARG | GLU | ALA | GLY | ASP | VAL | CYS | TYR | ALA | ||||
| 16 | ASP | VAL | TYR | ARG | ASP | GLY | THR | GLY | VAL | VAL | ||||
| 17 | GLU | PHE | VAL | ARG | LYS | GLU | ASP | MET | THR | TYR | ||||
| 18 | ALA | VAL | ARG | LYS | LEU | ASP | ASN | THR | LYS | PHE | ||||
| 19 | ARG | SER | HIS | GLU | GLY | GLU | THR | ALA | TYR | ILE | ||||
| 20 | ARG | VAL | LYS | VAL | ASP | GLY |
Entity 2, RNA 8 residues - Formula weight is not available
| 1 | U | C | A | G | A | G | G | A |
Samples:
sample_1: SRSF1_1-196, [U-13C; U-15N; U-2H], 436 uM; Single stranded 8mer RNA 620 uM; MES 20 mM; Arg/Glu Acid 380 mM
sample_conditions_1: pH: 6.2; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III HD 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts