BMRB Entry 51259

Name: Islet amyloid polypeptide (amylin) in solution pH 6.5 10degC
Published: 2022-01-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51259

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Islet amyloid polypeptide (amylin) in solution pH 6.5 10degC PubMed: 35501361

Deposition date: 2022-01-05 Original release date: 2022-01-20

Authors: Torner, Ricarda; Kupreichyk, Tatsiana; Gremer, Lothar; Colas Debled, Elisa; Fenel, Dapna; Gans, Pierre; Willbold, Dieter; Schoehn, Guy; Hoyer, Wolfgang; Boisbouvier, Jerome

Citation: Torner, Ricarda; Kupreichyk, Tatsiana; Gremer, Lothar; Debled, Elisa Colas; Fenel, Daphna; Schemmert, Sarah; Gans, Pierre; Willbold, Dieter; Schoehn, Guy; Hoyer, Wolfgang; Boisbouvier, Jerome. "Structural basis for the inhibition of IAPP fibril formation by the co-chaperonin prefoldin" Nat. Commun. 13, 2363-2363 (2022).

Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET302/NT-His

Entity Sequences (FASTA):
entity_1: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	103
15N chemical shifts	34
1H chemical shifts	34

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hIAPP	1

Entities:

Entity 1, hIAPP 37 residues - Formula weight is not available

1

LYS

CYS

ASN

THR

ALA

THR

CYS

ALA

THR

GLN

2

ARG

LEU

ALA

ASN

PHE

LEU

VAL

HIS

SER

3

ASN

PHE

GLY

ALA

ILE

LEU

SER

THR

4

ASN

VAL

GLY

SER

ASN

THR

TYR

Samples:

sample_1: human Islet amyloid polypeptide (hIAPP), [U-100% 13C; U-100% 15N], 80 uM; MES/NaOH 25 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts