BMRB Entry 51286

Name: CLAMP-interacting disordered region of male-specific lethal protein 2 (Msl2)
Published: 2022-02-02

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51286

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: CLAMP-interacting disordered region of male-specific lethal protein 2 (Msl2) PubMed: 35648444

Deposition date: 2022-01-21 Original release date: 2022-02-02

Authors: Mariasina, Sofia; Bonchuk, Artem; Polshakov, Vladimir

Citation: Tihonova, Evgeniya; Mariasina, Sofia; Efimov, Sergey; Polshakov, Vladimir; Maksimenko, Oksana; Georgiev, Pavel; Bonchuk, Artem. "Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila" Nucleic Acids Res. 50, 6521-6531 (2022).

Assembly members:
entity_1, polymer, 42 residues, Formula weight is not available

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)

Entity Sequences (FASTA):
entity_1: SGSISLVPLNNLQQSQHPLV LVQNEKGEYQGFNIFQGSKP LD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	111
15N chemical shifts	38
1H chemical shifts	139

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Msl2	1

Entities:

Entity 1, Msl2 42 residues - Formula weight is not available

First three residues (S-615, G-616, and S-617) are parts of the affinity tag. The numbering used in the project corresponds to the whole protein (since I618).

1

SER

GLY

SER

ILE

SER

LEU

VAL

PRO

LEU

ASN

2

ASN

LEU

GLN

SER

GLN

HIS

PRO

LEU

VAL

3

LEU

VAL

GLN

ASN

GLU

LYS

GLY

GLU

TYR

GLN

4

GLY

PHE

ASN

ILE

PHE

GLN

GLY

SER

LYS

PRO

5

LEU

ASP

Samples:

sample_1: Msl2, [U-98% 13C; U-98% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 50 mM; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 50 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - data analysis

TOPSPIN - collection

PINE - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts