BMRB Entry 51299

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51299

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Title:
SRSF1 Unphosphorylated
Deposition date:
2022-02-02
Original release date:
2023-03-21
Authors:
Fargason, Talia; Zhang, Jun
Citation:

Citation: Fargason, Talia; De Silva, Naiduwadura Ivon Upekala; King, Erin; Zhang, Zihan; Paul, Trenton; Shariq, Jamal; Zaharias, Steve; Zhang, Jun. "Peptides that Mimic RS repeats modulate phase separation of SRSF1, revealing a reliance on combined stacking and electrostatic interactions"  Elife 12, e84412-e84412 (2023).
PubMed: 36862748

Assembly members:

Assembly members:
entity_1, polymer, 248 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PSMT3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSGGGVIRGPAGNNDCRIYV GNLPPDIRTKDIEDVFYKYG AIRDIDLKNRRGGPPFAFVE FEDPRDAEDAVYGRDGYDYD GYRLRVEFPRSGRGTGRGGG GGGGGGAPRGRYGPPSRRSE NRVVVSGLPPSGSWQDLKDH MREAGDVCYADVYRDGTGVV EFVRKEDMTYAVRKLDNTKF RSHEGETAYIRVKVDGPRSP SYGRSRSRSRSRSRSRSRSN SRSRSYSPRRSRGSPRYSPR HSRSRSRT

Data sets:
Data typeCount
13C chemical shifts580
15N chemical shifts191
1H chemical shifts192

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SRSF11

Entities:

Entity 1, SRSF1 248 residues - Formula weight is not available

1   METSERGLYGLYGLYVALILEARGGLYPRO
2   ALAGLYASNASNASPCYSARGILETYRVAL
3   GLYASNLEUPROPROASPILEARGTHRLYS
4   ASPILEGLUASPVALPHETYRLYSTYRGLY
5   ALAILEARGASPILEASPLEULYSASNARG
6   ARGGLYGLYPROPROPHEALAPHEVALGLU
7   PHEGLUASPPROARGASPALAGLUASPALA
8   VALTYRGLYARGASPGLYTYRASPTYRASP
9   GLYTYRARGLEUARGVALGLUPHEPROARG
10   SERGLYARGGLYTHRGLYARGGLYGLYGLY
11   GLYGLYGLYGLYGLYGLYALAPROARGGLY
12   ARGTYRGLYPROPROSERARGARGSERGLU
13   ASNARGVALVALVALSERGLYLEUPROPRO
14   SERGLYSERTRPGLNASPLEULYSASPHIS
15   METARGGLUALAGLYASPVALCYSTYRALA
16   ASPVALTYRARGASPGLYTHRGLYVALVAL
17   GLUPHEVALARGLYSGLUASPMETTHRTYR
18   ALAVALARGLYSLEUASPASNTHRLYSPHE
19   ARGSERHISGLUGLYGLUTHRALATYRILE
20   ARGVALLYSVALASPGLYPROARGSERPRO
21   SERTYRGLYARGSERARGSERARGSERARG
22   SERARGSERARGSERARGSERARGSERASN
23   SERARGSERARGSERTYRSERPROARGARG
24   SERARGGLYSERPROARGTYRSERPROARG
25   HISSERARGSERARGSERARGTHR

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SPARKY: Backbone or all simulated peaks