BMRB Entry 51301
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51301
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Title: Backbone and side chain resonance assignment of the intrinsically disordered human DBNDD1 protein PubMed: 35474152
Deposition date: 2022-02-04 Original release date: 2022-05-27
Authors: Obika, Kingsley Benjamin; Liebscher, Sandra; Jirscitzka, Jan; Ohlenschlager, Oliver; Bordusa, Frank; Wiedemann, Christoph
Citation: Wiedemann, Christoph; Obika, Kingsley Benjamin; Liebscher, Sandra; Jirschitzka, Jan; Ohlenschlager, Oliver; Bordusa, Frank. "Backbone and side chain resonance assignment of the intrinsically disordered human DBNDD1 protein" Biomol. NMR Assignments 16, 237-246 (2022).
Assembly members:
entity_1, polymer, 178 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH
MEPPEGAGTGEIVKEAEVPQ
AALGVPAQGTGDNGHTPVEE
EVGGIPVPAPGLLQVTERRQ
PLSSVSSLEVHFDLLDLTEL
TDMSDQELAEVFADSDDENL
NTESPAGLHPLPRAGYLRSP
SWTRTRAEQSHEKQPLGDPE
RQATVLDTFLTVERPQED
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 667 |
| 15N chemical shifts | 171 |
| 1H chemical shifts | 903 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human DBNDD1 | 1 |
Entities:
Entity 1, human DBNDD1 178 residues - Formula weight is not available
Purification tag N-terminal to natural sequence, purification tag numbered from -19-0.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | GLU | PRO | PRO | GLU | GLY | ALA | GLY | THR | GLY | ||||
| 4 | GLU | ILE | VAL | LYS | GLU | ALA | GLU | VAL | PRO | GLN | ||||
| 5 | ALA | ALA | LEU | GLY | VAL | PRO | ALA | GLN | GLY | THR | ||||
| 6 | GLY | ASP | ASN | GLY | HIS | THR | PRO | VAL | GLU | GLU | ||||
| 7 | GLU | VAL | GLY | GLY | ILE | PRO | VAL | PRO | ALA | PRO | ||||
| 8 | GLY | LEU | LEU | GLN | VAL | THR | GLU | ARG | ARG | GLN | ||||
| 9 | PRO | LEU | SER | SER | VAL | SER | SER | LEU | GLU | VAL | ||||
| 10 | HIS | PHE | ASP | LEU | LEU | ASP | LEU | THR | GLU | LEU | ||||
| 11 | THR | ASP | MET | SER | ASP | GLN | GLU | LEU | ALA | GLU | ||||
| 12 | VAL | PHE | ALA | ASP | SER | ASP | ASP | GLU | ASN | LEU | ||||
| 13 | ASN | THR | GLU | SER | PRO | ALA | GLY | LEU | HIS | PRO | ||||
| 14 | LEU | PRO | ARG | ALA | GLY | TYR | LEU | ARG | SER | PRO | ||||
| 15 | SER | TRP | THR | ARG | THR | ARG | ALA | GLU | GLN | SER | ||||
| 16 | HIS | GLU | LYS | GLN | PRO | LEU | GLY | ASP | PRO | GLU | ||||
| 17 | ARG | GLN | ALA | THR | VAL | LEU | ASP | THR | PHE | LEU | ||||
| 18 | THR | VAL | GLU | ARG | PRO | GLN | GLU | ASP |
Samples:
sample_1: human DBNDD1, [U-99% 13C; U-99% 15N], 300 uM; DSS 100 uM; sodium phosphate 10 mM; sodium chloride 150 mM
sample_2: human DBNDD1, [U-99% 13C; U-99% 15N], 300 uM; DSS 100 uM; sodium phosphate 10 mM; sodium chloride 150 mM
sample_conditions_1: ionic strength: 0.16 M; pH: 6.5; pressure: 1 atm; temperature: 283.2 K
sample_conditions_2: ionic strength: 0.16 M; pH: 6.5; pressure: 1 atm; temperature: 293.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D CON | sample_2 | isotropic | sample_conditions_2 |
| (H)CACO | sample_2 | isotropic | sample_conditions_2 |
| (H)CBCACON | sample_2 | isotropic | sample_conditions_2 |
| (H)CBCANCO | sample_2 | isotropic | sample_conditions_2 |
Software:
CcpNMR - chemical shift assignment
TOPSPIN v3.6.2 - collection, processing
NMRbox - data analysis
NMR spectrometers:
- Bruker AVANCE III 700.5 MHz
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts