BMRB Entry 51304
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51304
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Title: Backbone assignment for human Cdc20 (1-73) PubMed: 36289199
Deposition date: 2022-02-05 Original release date: 2022-09-09
Authors: Fischer, Elyse; Yu, Conny; Hevler, Johannes; McLaughlin, Stephen; Tamara, Sem; Maslen, Sarah; Cowieson, Nathan; Heck, Albert; Freund, Stefan; Barford, David
Citation: Fischer, Elyse; Yu, Conny; Hevler, Johannes; McLaughlin, Stephen; Maslen, Sarah; Heck, Albert; Freund, Stefan; Barford, David. "Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly" Nat. Commun. 13, 6381-6381 (2022).
Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSFDuet-1
Entity Sequences (FASTA):
entity_1: MAQFAFESDLHSLLQLDAPI
PNAPPARWQRKAKEAAGPAP
SPMRAANRSHSAGRTPGRTP
GKSSSKVQTTPSK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 215 |
15N chemical shifts | 72 |
1H chemical shifts | 57 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Cdc20(1-73) | 1 |
Entities:
Entity 1, Cdc20(1-73) 73 residues - Formula weight is not available
1 | MET | ALA | GLN | PHE | ALA | PHE | GLU | SER | ASP | LEU | ||||
2 | HIS | SER | LEU | LEU | GLN | LEU | ASP | ALA | PRO | ILE | ||||
3 | PRO | ASN | ALA | PRO | PRO | ALA | ARG | TRP | GLN | ARG | ||||
4 | LYS | ALA | LYS | GLU | ALA | ALA | GLY | PRO | ALA | PRO | ||||
5 | SER | PRO | MET | ARG | ALA | ALA | ASN | ARG | SER | HIS | ||||
6 | SER | ALA | GLY | ARG | THR | PRO | GLY | ARG | THR | PRO | ||||
7 | GLY | LYS | SER | SER | SER | LYS | VAL | GLN | THR | THR | ||||
8 | PRO | SER | LYS |
Samples:
sample_1: Cdc20(1-73), [U-13C; U-15N; U-2H], 200 uM; HEPES 20 mM; sodium chloride 100 mM; TCEP 1 mM; sodium azide 0.02%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
2D 13C-detected CON | sample_1 | isotropic | sample_conditions_1 |
3D 13C-detected CBCACON | sample_1 | isotropic | sample_conditions_1 |
3D 13C-detected CBCANCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.1.1 - collection, processing
NMRFAM-SPARKY v1.47 - chemical shift assignment, data analysis, peak picking
MddNMR - processing
MARS - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE II+ 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts