BMRB Entry 51314

Name: human FUS 454-526
Published: 2022-03-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51314

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: human FUS 454-526 PubMed: 35203243

Deposition date: 2022-02-08 Original release date: 2022-03-28

Authors: Lenard, Aneta; Bourgeois, Benjamin; Zhou, Qishun; Madl, Tobias

Citation: Lenard, Aneta; Zhou, Qishun; Madreiter-Sokolowski, Corina; Bourgeois, Benjamin; Habacher, Hermann; Khanna, Yukti; Madl, Tobias. "EGCG Promotes FUS Condensate Formation in a Methylation-Dependent Manner" Cells 11, 592-592 (2022).

Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):
entity_1: GPGGGPGGSHMGGNYGDDRR GGRGGYDRGGYRGRGGDRGG FRGGRGGGDRGGFGPGKMDS RGEHRQDRRERPY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	115
15N chemical shifts	67
1H chemical shifts	68

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human FUS 454-526	1

Entities:

Entity 1, human FUS 454-526 73 residues - Formula weight is not available

1

GLY

PRO

GLY

PRO

GLY

SER

HIS

2

MET

GLY

ASN

TYR

GLY

ASP

ARG

3

GLY

ARG

GLY

TYR

ASP

ARG

GLY

4

TYR

ARG

GLY

ARG

GLY

ASP

ARG

GLY

5

PHE

ARG

GLY

ARG

GLY

ASP

ARG

6

GLY

PHE

GLY

PRO

GLY

LYS

MET

ASP

SER

7

ARG

GLY

GLU

HIS

ARG

GLN

ASP

ARG

GLU

8

ARG

PRO

TYR

Samples:

sample_1: Fused-In-Sarcoma, [U-100% 13C; U-100% 15N], 583 uM; succinic acid 50 mM; sodium chloride 150 mM; TCEP 2 mM; sodium azide 0.04%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
HNCANNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4 - chemical shift assignment, data analysis, peak picking, processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts