BMRB Entry 51318
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51318
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Title: C-terminal domain of the Vasopressin V2 receptor PubMed: 35625550
Deposition date: 2022-02-09 Original release date: 2022-06-08
Authors: Guillien, Myriam; Sibille, Nathalie
Citation: Guillien, Myriam; Mouhand, Assia; Fournet, Aurelie; Gontier, Amandine; Marti Navia, Aleix; Cordeiro, Tiago; Allemand, Frederic; Thureau, Aurelien; Baneres, Jean-Louis; Bernado, Pau; Sibille, Nathalie. "Structural Insights into the Intrinsically Disordered GPCR C-Terminal Region, Major Actor in Arrestin-GPCR Interaction" Biomolecules 12, 617-617 (2022).
Assembly members:
entity_1, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM33
Entity Sequences (FASTA):
entity_1: GPMAARGRTPPSLGPQDESC
TTASSSLAKDTSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 82 |
| 15N chemical shifts | 28 |
| 1H chemical shifts | 28 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | V2R-Cter GPCR | 1 |
Entities:
Entity 1, V2R-Cter GPCR 33 residues - Formula weight is not available
| 1 | GLY | PRO | MET | ALA | ALA | ARG | GLY | ARG | THR | PRO | ||||
| 2 | PRO | SER | LEU | GLY | PRO | GLN | ASP | GLU | SER | CYS | ||||
| 3 | THR | THR | ALA | SER | SER | SER | LEU | ALA | LYS | ASP | ||||
| 4 | THR | SER | SER |
Samples:
sample_1: V2R-Cter GPCR, [U-13C; U-15N], 170 uM; Bis-Tris 50 mM; NaCl 150 mM; EDTA 1 mM; TCEP 0.5 mM; DSS-d6, [U-2H], 5 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 2D HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts