BMRB Entry 5132
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5132
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Title: 1H and 15N assignments of ATTm+4, a truncated version of ATT, an trypsin/chymotrypsin inhibitor from Arabidopsis thaliana PubMed: 12369816
Deposition date: 2001-09-06 Original release date: 2003-06-10
Authors: Zhao, Qin; Chae, Young-Kee; Markley, John
Citation: Zhao, Qin; Chae, Young-Kee; Markley, John. "NMR Solution Structure of ATTp, an Arabidopsis thaliana Trypsin Inhibitor" Biochemistry 41, 12284-12296 (2002).
Assembly members:
attm+4, polymer, 66 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPIC9k/attp
Entity Sequences (FASTA):
attm+4: YVEFQGNECLKEYGGDVGFG
FCAPRIFPTICYTRCRENKG
AKGGRCRWGQGSNVKCLCDF
CGDTPQ
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 55 |
1H chemical shifts | 219 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | attm+4 | 1 |
Entities:
Entity 1, attm+4 66 residues - Formula weight is not available
1 | TYR | VAL | GLU | PHE | GLN | GLY | ASN | GLU | CYS | LEU | ||||
2 | LYS | GLU | TYR | GLY | GLY | ASP | VAL | GLY | PHE | GLY | ||||
3 | PHE | CYS | ALA | PRO | ARG | ILE | PHE | PRO | THR | ILE | ||||
4 | CYS | TYR | THR | ARG | CYS | ARG | GLU | ASN | LYS | GLY | ||||
5 | ALA | LYS | GLY | GLY | ARG | CYS | ARG | TRP | GLY | GLN | ||||
6 | GLY | SER | ASN | VAL | LYS | CYS | LEU | CYS | ASP | PHE | ||||
7 | CYS | GLY | ASP | THR | PRO | GLN |
Samples:
sample_1: attm+4, [U-15N], 1 mM; sodium acetate, [U-2H], 50 mM; DSS 0.1 mM
Ex-cond: pH: 5.0; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
15N HSQC | not available | not available | not available |
15N TOCSY | not available | not available | not available |
15N NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 500 MHz
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SPARKY: Backbone
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