BMRB Entry 51325
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51325
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NMR-STAR v3 text file.
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Title: N-terminal domain of SARS-CoV-2 Nsp8 protein PubMed: 37665006
Deposition date: 2022-02-12 Original release date: 2022-02-21
Authors: Trevino, Miguel; Pantoja-Uceda, David; Laurents, Douglas; Mompean, Miguel
Citation: Trevino, Miguel; Pantoja-Uceda, David; Laurents, Douglas; Mompean, Miguel. "SARS-CoV-2 Nsp8 N-terminal domain folds autonomously and binds dsRNA" Nucleic Acids Res. 51, 10041-10048 (2023).
Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
entity_1: AIASEFSSLPSYAAFATAQE
AYEQAVANGDSEVVLKKLKK
SLNVAKSEFDRDAAMQRKLE
KMADQAMTQMYKQARSEDKR
AKVT
- assigned_chemical_shifts
- coupling_constants
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T1rho_relaxation
| Data type | Count |
| 13C chemical shifts | 306 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 534 |
| T1 relaxation values | 79 |
| T1rho relaxation values | 79 |
| coupling constants | 64 |
| heteronuclear NOE values | 79 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nsp8 NTD, chain 1 | 1 |
| 2 | Nsp8 NTD, chain 2 | 1 |
Entities:
Entity 1, Nsp8 NTD, chain 1 84 residues - Formula weight is not available
| 1 | ALA | ILE | ALA | SER | GLU | PHE | SER | SER | LEU | PRO | ||||
| 2 | SER | TYR | ALA | ALA | PHE | ALA | THR | ALA | GLN | GLU | ||||
| 3 | ALA | TYR | GLU | GLN | ALA | VAL | ALA | ASN | GLY | ASP | ||||
| 4 | SER | GLU | VAL | VAL | LEU | LYS | LYS | LEU | LYS | LYS | ||||
| 5 | SER | LEU | ASN | VAL | ALA | LYS | SER | GLU | PHE | ASP | ||||
| 6 | ARG | ASP | ALA | ALA | MET | GLN | ARG | LYS | LEU | GLU | ||||
| 7 | LYS | MET | ALA | ASP | GLN | ALA | MET | THR | GLN | MET | ||||
| 8 | TYR | LYS | GLN | ALA | ARG | SER | GLU | ASP | LYS | ARG | ||||
| 9 | ALA | LYS | VAL | THR |
Samples:
sample_1: molecule_1, [U-100% 13C; U-100% 15N], 100 uM; NaCl 50 mM; KH2PO4 10 mM
sample_conditions_1: ionic strength: 60 mM; pH: 6.1; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| hsqct1etf3gpsi | sample_1 | isotropic | sample_conditions_1 |
| hsqctref3gpsi | sample_1 | isotropic | sample_conditions_1 |
| hsqcnoef3gpsi | sample_1 | isotropic | sample_conditions_1 |
| hnhagp3d | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts