BMRB Entry 51341

Name: NMR Assignment of Backbone (1H, 15N, and 13C) resonances of Histone Like DNA binding protein of Helicobacter pylori (Hup-P64A) variant
Published: 2022-05-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51341

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR Assignment of Backbone (1H, 15N, and 13C) resonances of Histone Like DNA binding protein of Helicobacter pylori (Hup-P64A) variant PubMed: 35572751

Deposition date: 2022-03-01 Original release date: 2022-05-27

Authors: Agarwal, Nipanshu; Kumar, Dinesh; Poluri, Krishna Mohan

Citation: Agarwal, Nipanshu; Nagar, Nupur; Raj, Ritu; Kumar, Dinesh; Poluri, Krishna Mohan. "Conserved Apical Proline Regulating the Structure and DNA Binding Properties of Helicobacter pylori Histone-like DNA Binding Protein (Hup)" ACS Omega 7, 15231-15246 (2022).

Assembly members:
entity_1, polymer, 98 residues, 10772 Da.

Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE 80L

Entity Sequences (FASTA):
entity_1: GAMEMNKAEFIDLVKEAGKY NSKREAEEAISAFTLAVETA LSKGESVELIGFGKFETAEQ KGKEGKVAGSDKTYKTEDKR VPKFKPGKTLKQKVEEGK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	252
15N chemical shifts	86
1H chemical shifts	86

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Chain A	1
2	Chain B	1

Entities:

Entity 1, Chain A 98 residues - 10772 Da.

Residue -4 to -1 represent cloning artifacts Protein has 6X-His tag at N terminus

1

GLY

ALA

MET

GLU

MET

ASN

LYS

ALA

GLU

PHE

2

ILE

ASP

LEU

VAL

LYS

GLU

ALA

GLY

LYS

TYR

3

ASN

SER

LYS

ARG

GLU

ALA

GLU

ALA

ILE

4

SER

ALA

PHE

THR

LEU

ALA

VAL

GLU

THR

ALA

5

LEU

SER

LYS

GLY

GLU

SER

VAL

GLU

LEU

ILE

6

GLY

PHE

GLY

LYS

PHE

GLU

THR

ALA

GLU

GLN

7

LYS

GLY

LYS

GLU

GLY

LYS

VAL

ALA

GLY

SER

8

ASP

LYS

THR

TYR

LYS

THR

GLU

ASP

LYS

ARG

9

VAL

PRO

LYS

PHE

LYS

PRO

GLY

LYS

THR

LEU

10

LYS

GLN

LYS

VAL

GLU

GLY

LYS

Samples:

sample_1: Histone Like DNA binding protein of Helicobacter pylori (Hup-P64A) variant, [U-99% 13C; U-99% 15N], 0.75 mM; D2O, [U-100% 15N], 10%; sodium chloride 200 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 0.079 M; pH: 6; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1 - chemical shift assignment, data analysis

TOPSPIN v3.6.1 - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts