BMRB Entry 51342
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PDB ID: 8atk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51342
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Fowler, Nicholas; Albalwi, Marym; Lee, Subin; Hounslow, Andrea; Williamson, Mike. "Improved methodology for protein NMR structure calculation using hydrogen bond restraints and ANSURR validation: The SH2 domain of SH2B1" Structure 31, 975-986 (2023).
PubMed: 37311460
Assembly members:
entity_1, polymer, 119 residues, 13664 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-4
Entity Sequences (FASTA):
entity_1: MKIHHHHHHDQPLSGYPWFH
GMLSRLKAAQLVLEGGTGSH
GVFLVRQSETRRGEYVLTFN
FQGKAKHLRLSLNEEGQCRV
QHLWFQSIFDMLEHFRVHPI
PLESGGSSDVVLVSYVPSQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 541 |
| 15N chemical shifts | 140 |
| 1H chemical shifts | 843 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH2 domain | 1 |
Entities:
Entity 1, SH2 domain 119 residues - 13664 Da.
This sequence corresponds to residues 519-628 from mouse SH2B1 (Uniprot Q91ZM2) with the addition of a 9-residue His tag at the N terminus. Thus the first included residue of the mouse sequence (D519) is Asp-10.
| 1 | MET | LYS | ILE | HIS | HIS | HIS | HIS | HIS | HIS | ASP | ||||
| 2 | GLN | PRO | LEU | SER | GLY | TYR | PRO | TRP | PHE | HIS | ||||
| 3 | GLY | MET | LEU | SER | ARG | LEU | LYS | ALA | ALA | GLN | ||||
| 4 | LEU | VAL | LEU | GLU | GLY | GLY | THR | GLY | SER | HIS | ||||
| 5 | GLY | VAL | PHE | LEU | VAL | ARG | GLN | SER | GLU | THR | ||||
| 6 | ARG | ARG | GLY | GLU | TYR | VAL | LEU | THR | PHE | ASN | ||||
| 7 | PHE | GLN | GLY | LYS | ALA | LYS | HIS | LEU | ARG | LEU | ||||
| 8 | SER | LEU | ASN | GLU | GLU | GLY | GLN | CYS | ARG | VAL | ||||
| 9 | GLN | HIS | LEU | TRP | PHE | GLN | SER | ILE | PHE | ASP | ||||
| 10 | MET | LEU | GLU | HIS | PHE | ARG | VAL | HIS | PRO | ILE | ||||
| 11 | PRO | LEU | GLU | SER | GLY | GLY | SER | SER | ASP | VAL | ||||
| 12 | VAL | LEU | VAL | SER | TYR | VAL | PRO | SER | GLN |
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