BMRB Entry 51343
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51343
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Title: TrkA_eJTM_TMD_wt PubMed: 35601915
Deposition date: 2022-03-02 Original release date: 2022-05-27
Authors: Kot, Erik; Vasilieva, Ekaterina; Shabalkina, Alexandra; Goncharuk, Sergey; Mineev, Konstantin
Citation: Kot, Erik; Franco, Maria; Vasilieva, Ekaterina; Shabalkina, Alexandra; Arseniev, Alexander; Goncharuk, Sergey; Mineev, Konstantin; Vilar, Marcal. "Intrinsically disordered regions couple the ligand binding and kinase activation of Trk neurotrophin receptors" iScience 25, 104348-104348 (2022).
Assembly members:
entity_1, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: Escherichia coli Vector: pGEMEX-1/H6tag
Entity Sequences (FASTA):
entity_1: GSDNPFEFNPEDPIPVSFSP
VDTNSTSGDPVEKKDETPFG
VSVAVGLAVFACLFLSTLLL
VLNKAGRRNK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 161 |
| 15N chemical shifts | 58 |
| 1H chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TrkA-eJTM-TMD | 1 |
Entities:
Entity 1, TrkA-eJTM-TMD 70 residues - Formula weight is not available
| 1 | GLY | SER | ASP | ASN | PRO | PHE | GLU | PHE | ASN | PRO | |
| 2 | GLU | ASP | PRO | ILE | PRO | VAL | SER | PHE | SER | PRO | |
| 3 | VAL | ASP | THR | ASN | SER | THR | SER | GLY | ASP | PRO | |
| 4 | VAL | GLU | LYS | LYS | ASP | GLU | THR | PRO | PHE | GLY | |
| 5 | VAL | SER | VAL | ALA | VAL | GLY | LEU | ALA | VAL | PHE | |
| 6 | ALA | CYS | LEU | PHE | LEU | SER | THR | LEU | LEU | LEU | |
| 7 | VAL | LEU | ASN | LYS | ALA | GLY | ARG | ARG | ASN | LYS |
Samples:
sample_1: TrkA-eJTM-TMD, [U-100% 13C; U-100% 15N], 0.30 mM; DMPC 21.5 mM; CHAPS 49.9 mM; Sodium phosphate buffer 50 mM; D2O, [U-2H], 5 % v/v; NaN3 0.01%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.4; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCA | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts