BMRB Entry 51359
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51359
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Title: Backbone resonance assignments of the N-terminal domain of Sam68 PubMed: 36537190
Deposition date: 2022-03-11 Original release date: 2022-11-23
Authors: Malki, Idir; Dominguez, Cyril
Citation: Malki, Idir; Liepina, Inara; Kogelnik, Nora; Watmuff, Hollie; Robinson, Sue; Lightfoot, Adam; Gonchar, Oksana; Bottrill, Andrew; Fry, Andrew; Dominguez, Cyril. "Cdk1-mediated threonine phosphorylation of Sam68 modulates its RNA binding, alternative splicing activity and cellular functions" Nucleic Acids Res. 50, 13045-13062 (2022).
Assembly members:
entity_1, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLEICS-01
Entity Sequences (FASTA):
entity_1: SMQRRDDPAARMSRSSGRSG
SMDPSGAHPSVRQTPSRQPP
LPHRSRGGGGGSRGGARASP
ATQPPPLLPPSATGPDATVG
GPAPTPLLPPSATASVK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 91 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 50 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sam68 N-terminal domain | 1 |
Entities:
Entity 1, Sam68 N-terminal domain 97 residues - Formula weight is not available
| 1 | SER | MET | GLN | ARG | ARG | ASP | ASP | PRO | ALA | ALA | ||||
| 2 | ARG | MET | SER | ARG | SER | SER | GLY | ARG | SER | GLY | ||||
| 3 | SER | MET | ASP | PRO | SER | GLY | ALA | HIS | PRO | SER | ||||
| 4 | VAL | ARG | GLN | THR | PRO | SER | ARG | GLN | PRO | PRO | ||||
| 5 | LEU | PRO | HIS | ARG | SER | ARG | GLY | GLY | GLY | GLY | ||||
| 6 | GLY | SER | ARG | GLY | GLY | ALA | ARG | ALA | SER | PRO | ||||
| 7 | ALA | THR | GLN | PRO | PRO | PRO | LEU | LEU | PRO | PRO | ||||
| 8 | SER | ALA | THR | GLY | PRO | ASP | ALA | THR | VAL | GLY | ||||
| 9 | GLY | PRO | ALA | PRO | THR | PRO | LEU | LEU | PRO | PRO | ||||
| 10 | SER | ALA | THR | ALA | SER | VAL | LYS |
Samples:
sample_1: N-terminal domain of Sam68, [U-99% 13C; U-99% 15N], 500 uM
sample_conditions_1: ionic strength: 0.25 M; pH: 7.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4.2 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts