BMRB Entry 51359

Name: Backbone resonance assignments of the N-terminal domain of Sam68
Published: 2022-11-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51359

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments of the N-terminal domain of Sam68

Deposition date:

2022-03-11

Original release date:

2022-11-23

Authors:

Malki, Idir; Dominguez, Cyril

Citation:

Citation: Malki, Idir; Liepina, Inara; Kogelnik, Nora; Watmuff, Hollie; Robinson, Sue; Lightfoot, Adam; Gonchar, Oksana; Bottrill, Andrew; Fry, Andrew; Dominguez, Cyril. "Cdk1-mediated threonine phosphorylation of Sam68 modulates its RNA binding, alternative splicing activity and cellular functions" Nucleic Acids Res. 50, 13045-13062 (2022).
PubMed: 36537190

Assembly members:

Assembly members:
entity_1, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLEICS-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMQRRDDPAARMSRSSGRSG SMDPSGAHPSVRQTPSRQPP LPHRSRGGGGGSRGGARASP ATQPPPLLPPSATGPDATVG GPAPTPLLPPSATASVK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	91
15N chemical shifts	50
1H chemical shifts	50

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sam68 N-terminal domain	1

Entities:

Entity 1, Sam68 N-terminal domain 97 residues - Formula weight is not available

1

SER

MET

GLN

ARG

ASP

PRO

ALA

2

ARG

MET

SER

ARG

SER

GLY

ARG

SER

GLY

3

SER

MET

ASP

PRO

SER

GLY

ALA

HIS

PRO

SER

4

VAL

ARG

GLN

THR

PRO

SER

ARG

GLN

PRO

5

LEU

PRO

HIS

ARG

SER

ARG

GLY

6

GLY

SER

ARG

GLY

ALA

ARG

ALA

SER

PRO

7

ALA

THR

GLN

PRO

LEU

PRO

8

SER

ALA

THR

GLY

PRO

ASP

ALA

THR

VAL

GLY

9

GLY

PRO

ALA

PRO

THR

PRO

LEU

PRO

10

SER

ALA

THR

ALA

SER

VAL

LYS

Samples:

sample_1: N-terminal domain of Sam68, [U-99% 13C; U-99% 15N], 500 uM

sample_conditions_1: ionic strength: 0.25 M; pH: 7.0; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks