BMRB Entry 51376

Name: Backbone chemical shifts of Spd1
Published: 2022-09-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51376

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts of Spd1

Deposition date:

2022-03-21

Original release date:

2022-09-13

Authors:

Broendum, Sebastian; Prestel, Andreas; Kragelund, Birthe

Citation:

Citation: Dreier, Jesper; Prestel, Andreas; Martins, Joao; Brondum, Sebastian; Nielsen, Olaf; Garbers, Anna; Suga, Hiroaki; Boomsma, Wouter; Rogers, Joseph; Hartmann-Petersen, Rasmus; Kragelund, Birthe. "A context-dependent and disordered ubiquitin-binding motif" Cell. Mol. Life Sci. 79, 484-484 (2022).
PubMed: 35974206

Assembly members:

Assembly members:
Spd1, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Spd1: MHSSKRVMTTKTHVEQPESS MRPQLPESIQGSLMDVGMRV RKSISTGYKSKQTTFPAYNP PLYNTVSENIALKNTAFSYE PNGTKRPFEQAIPNYNWANP PQDFEEPEWLKPFDVVMEGT NERL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	344
15N chemical shifts	103
1H chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Spd1	1

Entities:

Entity 1, Spd1 124 residues - Formula weight is not available

1

MET

HIS

SER

LYS

ARG

VAL

MET

THR

2

LYS

THR

HIS

VAL

GLU

GLN

PRO

GLU

SER

3

MET

ARG

PRO

GLN

LEU

PRO

GLU

SER

ILE

GLN

4

GLY

SER

LEU

MET

ASP

VAL

GLY

MET

ARG

VAL

5

ARG

LYS

SER

ILE

SER

THR

GLY

TYR

LYS

SER

6

LYS

GLN

THR

PHE

PRO

ALA

TYR

ASN

PRO

7

PRO

LEU

TYR

ASN

THR

VAL

SER

GLU

ASN

ILE

8

ALA

LEU

LYS

ASN

THR

ALA

PHE

SER

TYR

GLU

9

PRO

ASN

GLY

THR

LYS

ARG

PRO

PHE

GLU

GLN

10

ALA

ILE

PRO

ASN

TYR

ASN

TRP

ALA

ASN

PRO

11

PRO

GLN

ASP

PHE

GLU

PRO

GLU

TRP

LEU

12

LYS

PRO

PHE

ASP

VAL

MET

GLU

GLY

THR

13

ASN

GLU

ARG

LEU

Samples:

sample_1: Spd1, [U-99% 13C; U-99% 15N], 50 ± 1 uM; DSS 250 uM; sodium chloride 100 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 120 mM; pH: 7.4; pressure: 1 atm; temperature: 277.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS v2.5 - chemical shift assignment

NMRDraw v9.9 - processing

NMR spectrometers:

Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks