BMRB Entry 51389
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51389
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Title: 1H and 13C chemical shifts for a peptide encompassing residues 2-19 of the human formin INF2 in aqueous solution PubMed: 36306014
Deposition date: 2022-04-06 Original release date: 2022-10-31
Authors: Jimenez, M. Angeles
Citation: Labat-de-Hoz, Leticia; Comas, Laura; Rubio-Ramos, Armando; Casares-Arias, Javier; Fernandez-Martin, Laura; Pantoja-Uceda, David; Martin, M. Teresa; Kremer, Leonor; Jimenez, M. Angeles; Correas, Isabel; Alonso, Miguel. "Structure and function of the N-terminal extension of the formin INF2" Cell. Mol. Life Sci. 79, 571-571 (2022).
Assembly members:
entity_1, polymer, 19 residues, 1998.38 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: SVKEGAQRKWAALKEKLGX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 124 |
| 1H chemical shifts | 269 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | INF2(2-19) | 1 |
Entities:
Entity 1, INF2(2-19) 19 residues - 1998.38 Da.
C-end amidated The C-terminal X corresponds to the C-terminal amide group
| 1 | SER | VAL | LYS | GLU | GLY | ALA | GLN | ARG | LYS | TRP | ||||
| 2 | ALA | ALA | LEU | LYS | GLU | LYS | LEU | GLY | NH2 |
Samples:
sample_1: INF2(2-19) 1 mM; D2O, [U-2H], 10 % v/v; H2O 90 % v/v; DSS 0.1 mM
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 278 K
sample_conditions_2: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 1D 1H | sample_1 | isotropic | sample_conditions_2 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_2 |
Software:
TOPSPIN - collection, processing
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz MHz
Related Database Links:
| NCBI | NP_071934 |