BMRB Entry 51401
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51401
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Title: SNAC-mEpoR-TM
Deposition date: 2022-04-17 Original release date: 2022-06-13
Authors: Mravic, Marco
Citation: Mravic, Marco. "Spectra of mEpoR transmembrane fragment in 2H-DPC, monomeric state" .
Assembly members:
entity_1, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
entity_1: SHHWNDPLILTLSLILVLIS
LLLTVLALLSHRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 117 |
| 15N chemical shifts | 31 |
| 1H chemical shifts | 241 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mEpoR-TM_monomer | 1 |
Entities:
Entity 1, mEpoR-TM_monomer 33 residues - Formula weight is not available
| 1 | SER | HIS | HIS | TRP | ASN | ASP | PRO | LEU | ILE | LEU | ||||
| 2 | THR | LEU | SER | LEU | ILE | LEU | VAL | LEU | ILE | SER | ||||
| 3 | LEU | LEU | LEU | THR | VAL | LEU | ALA | LEU | LEU | SER | ||||
| 4 | HIS | ARG | ARG |
Samples:
sample_1: mEpoR TM, [U-95% 13C; U-95% 15N], 1 mM; D2O 5%; H2O 95%; sodium chloride 20 mM; EDTA 1 mM; sodium acetate, [U-2H], 50 mM; DPC, [U-99% 2H], 400 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 5.2; pressure: 1 atm; temperature: 318.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
TOPSPIN v4.0.2 - collection
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts