BMRB Entry 51405

Name: NMR resonance assignments of the DNA binding domain of mouse Junctophilin-2
Published: 2022-07-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51405

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR resonance assignments of the DNA binding domain of mouse Junctophilin-2 PubMed: 35665900

Deposition date: 2022-04-20 Original release date: 2022-07-03

Authors: Yu, Liping; Hall, Duane; Zhao, Weiyang; Song, Long-Sheng

Citation: Yu, Liping; Hall, Duane; Zhao, Weiyang; Song, Long-Sheng. "NMR resonance assignments of the DNA binding domain of mouse Junctophilin-2" Biomol. NMR Assign. 16, 273-279 (2022).

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGEEGKYRH NVLVKGTKRRVLPLKSSKVR QKVEHGVEGAQRAAAIARQK AEIAASRTSHAKAKAEAAEQ AALAANQESNIARTLAWSHP QFEK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	378
15N chemical shifts	92
1H chemical shifts	584

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	JP2 DBD	1

Entities:

Entity 1, JP2 DBD 104 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

GLU

GLY

LYS

TYR

ARG

HIS

3

ASN

VAL

LEU

VAL

LYS

GLY

THR

LYS

ARG

4

VAL

LEU

PRO

LEU

LYS

SER

LYS

VAL

ARG

5

GLN

LYS

VAL

GLU

HIS

GLY

VAL

GLU

GLY

ALA

6

GLN

ARG

ALA

ILE

ALA

ARG

GLN

LYS

7

ALA

GLU

ILE

ALA

SER

ARG

THR

SER

HIS

8

ALA

LYS

ALA

LYS

ALA

GLU

ALA

GLU

GLN

9

ALA

LEU

ALA

ASN

GLN

GLU

SER

ASN

10

ILE

ALA

ARG

THR

LEU

ALA

TRP

SER

HIS

PRO

11

GLN

PHE

GLU

LYS

Samples:

sample_1: Junctophilin-2 (JP2) DNA-binding domain, [U-100% 13C; U-100% 15N], 0.8 mM; sodium chloride 40 mM; BisTris-HCl 20 mM

sample_conditions_1: ionic strength: 0.060 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe and NNRView - chemical shift assignment, data analysis, processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts