BMRB Entry 51419

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51419

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Title:
Backbone chemical shift assignments of R86A mutant of the Akt1 PH domain
Deposition date:
2022-04-26
Original release date:
2022-10-11
Authors:
Viennet, Thibault; Bae, Hwan; Chu, Nam; Cole, Philip; Arthanari, Haribabu
Citation:

Citation: Bae, Hwan; Viennet, Thibault; Park, Eunyoung; Chu, Nam; Salguero, Antonieta; Eck, Michael; Arthanari, Haribabu; Cole, Philip. "PH domain-mediated autoinhibition and oncogenic activation of Akt"  eLife 11, e80148-e80148 (2022).
PubMed: 35968932

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, 14436 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTXB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDVAIVKEGWLHKRGEYIK TWRPRYFLLKNDGTFIGYKE RPQDVDQREAPLNNFSVAQC QLMKTERPRPNTFIIRCLQW TTVIEATFHVETPEEREEWT TAIQTVADGLKKQEEEEMDF R

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts94
1H chemical shifts184

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Akt_PH_R86A1

Entities:

Entity 1, Akt_PH_R86A 121 residues - 14436 Da.

1   METSERASPVALALAILEVALLYSGLUGLY
2   TRPLEUHISLYSARGGLYGLUTYRILELYS
3   THRTRPARGPROARGTYRPHELEULEULYS
4   ASNASPGLYTHRPHEILEGLYTYRLYSGLU
5   ARGPROGLNASPVALASPGLNARGGLUALA
6   PROLEUASNASNPHESERVALALAGLNCYS
7   GLNLEUMETLYSTHRGLUARGPROARGPRO
8   ASNTHRPHEILEILEARGCYSLEUGLNTRP
9   THRTHRVALILEGLUALATHRPHEHISVAL
10   GLUTHRPROGLUGLUARGGLUGLUTRPTHR
11   THRALAILEGLNTHRVALALAASPGLYLEU
12   LYSLYSGLNGLUGLUGLUGLUMETASPPHE
13   ARG

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks