BMRB Entry 51421

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for T345D mutant of EZH2 loop
Published: 2022-06-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51421

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for T345D mutant of EZH2 loop

Deposition date: 2022-04-28 Original release date: 2022-06-03

Authors: Szabo, Csenge; Sebak, Fanni; Bodor, Andrea

Citation: Szabo, Csenge; Szabo, Beata; Sebak, Fanni; Bermel, Wolfgang; Tantos, Agnes; Bodor, Andrea. "The Disordered EZH2 Loop: Atomic Level Characterization by 1HN- and 1Ha-Detected NMR Approaches, Interaction with the Long Noncoding HOTAIR RNA" Int. J. Mol. Sci. 23, 6150-6150 (2022).

Assembly members:
entity_1, polymer, 106 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b (+)

Entity Sequences (FASTA):
entity_1: MDAERIKDPPKRPGGRRRGR LPNNSSRPSTPTINVLESKD TDSDREAGTETGGENNDKEE EEKKDETSSSSEANSRCQTP IKMKPNIEPPENVEWSGALE HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	238
15N chemical shifts	95
1H chemical shifts	302

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EZH2 T345D	1

Entities:

Entity 1, EZH2 T345D 106 residues - Formula weight is not available

Residues 338-339 and 436-443 represent cloning artifacts

1

MET

ASP

ALA

GLU

ARG

ILE

LYS

ASP

PRO

2

LYS

ARG

PRO

GLY

ARG

GLY

ARG

3

LEU

PRO

ASN

SER

ARG

PRO

SER

THR

4

PRO

THR

ILE

ASN

VAL

LEU

GLU

SER

LYS

ASP

5

THR

ASP

SER

ASP

ARG

GLU

ALA

GLY

THR

GLU

6

THR

GLY

GLU

ASN

ASP

LYS

GLU

7

GLU

LYS

ASP

GLU

THR

SER

8

SER

GLU

ALA

ASN

SER

ARG

CYS

GLN

THR

PRO

9

ILE

LYS

MET

LYS

PRO

ASN

ILE

GLU

PRO

10

GLU

ASN

VAL

GLU

TRP

SER

GLY

ALA

LEU

GLU

11

HIS

Samples:

sample_1: EZH2_T345D, [U-100% 13C; U-100% 15N], 1 mM; PBS 150 mM; TCEP 10 mM; sodium azide 3 mM; DSS 1%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D SHACA-HSCQ	sample_1	isotropic	sample_conditions_1
3D HCAN	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts