BMRB Entry 51427
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51427
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Title: B-Myb association with DNA is mediated by its negative regulatory domain and Cdk phosphorylation PubMed: 35926712
Deposition date: 2022-05-04 Original release date: 2022-10-19
Authors: Wijeratne, Tilini; Guiley, Keelan; Lee, Hsiau-Wei; Muller, Gerd; Rubin, Seth
Citation: Wijeratne, Tilini; Guiley, Keelan; Lee, Hsiau-Wei; Muller, Gerd; Rubin, Seth. "Cyclin-dependent kinase-mediated phosphorylation and the negative regulatory domain of transcription factor B-Myb modulate its DNA binding" J. Biol. Chem. 298, 102319-102319 (2022).
Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX
Entity Sequences (FASTA):
entity_1: KYSMDNTPHTPTPFKNALEK
YGPLKPLPQTPHLEEDLKEV
LRSEAGIELIIEDDIRPEKQ
KRKPGLRRSPIKKVRKSLAL
DIVDEDVKLMMST
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 257 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NRD | 1 |
Entities:
Entity 1, NRD 93 residues - Formula weight is not available
| 1 | LYS | TYR | SER | MET | ASP | ASN | THR | PRO | HIS | THR | ||||
| 2 | PRO | THR | PRO | PHE | LYS | ASN | ALA | LEU | GLU | LYS | ||||
| 3 | TYR | GLY | PRO | LEU | LYS | PRO | LEU | PRO | GLN | THR | ||||
| 4 | PRO | HIS | LEU | GLU | GLU | ASP | LEU | LYS | GLU | VAL | ||||
| 5 | LEU | ARG | SER | GLU | ALA | GLY | ILE | GLU | LEU | ILE | ||||
| 6 | ILE | GLU | ASP | ASP | ILE | ARG | PRO | GLU | LYS | GLN | ||||
| 7 | LYS | ARG | LYS | PRO | GLY | LEU | ARG | ARG | SER | PRO | ||||
| 8 | ILE | LYS | LYS | VAL | ARG | LYS | SER | LEU | ALA | LEU | ||||
| 9 | ASP | ILE | VAL | ASP | GLU | ASP | VAL | LYS | LEU | MET | ||||
| 10 | MET | SER | THR |
Samples:
sample_1: NRD, B-Myb, [U-100% 13C; U-100% 15N], 300 uM; sodium phosphate 20 mM; DTT 1 mM; sodium chloride 100 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CCONH | sample_1 | isotropic | sample_conditions_1 |
| 2D CON | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment
TOPSPIN - collection
VNMRj - collection
NMR spectrometers:
- Bruker AVANCE III HD 800 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts