BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 51431

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51431

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Title: ILV Methyl NMR Resonance Assignments of the 81 kDa E. coli b-clamp   PubMed: 35687262

Deposition date: 2022-05-06 Original release date: 2022-07-03

Authors: Lim, Socheata; Madhi, Sam; Beuning, Penny; Korzhnev, Dmitry

Citation: Lim, Socheata; Madhi, Sam; Beuning, Penny; Korzhnev, Dmitry. "ILV methyl NMR resonance assignments of the 81 kDa E. coli beta-clamp"  Biomol. NMR Assignments 16, 317-323 (2022).

Assembly members:
entity_1, polymer, 366 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b+

Entity Sequences (FASTA):
entity_1: MKFTVEREHLLKPLQQVSGP LGGRPTLPILGNLLLQVADG TLSLTGTDLEMEMVARVALV QPHEPGATTVPARKFFDICR GLPEGAEIAVQLEGERMLVR SGRSRFSLSTLPAADFPNLD DWQSEVEFTLPQATMKRLIE ATQFSMAHQDVRYYLNGMLF ETEGEELRTVATDGHRLAVC SMPIGQSLPSHSVIVPRKGV IELMRMLDGGDNPLRVQIGS NNIRAHVGDFIFTSKLVDGR FPDYRRVLPKNPDKHLEAGC DLLKQAFARAAILSNEKFRG VRLYVSENQLKITANNPEQE EAEEILDVTYSGAEMEIGFN VSYVLDVLNALKCENVRMML TDSVSSVQIEDAASQSAAYV VMPMRL

Data sets:
Data typeCount
13C chemical shifts159
1H chemical shifts477

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1The E. coli b-clamp1

Entities:

Entity 1, The E. coli b-clamp 366 residues - Formula weight is not available

1   METLYSPHETHRVALGLUARGGLUHISLEU
2   LEULYSPROLEUGLNGLNVALSERGLYPRO
3   LEUGLYGLYARGPROTHRLEUPROILELEU
4   GLYASNLEULEULEUGLNVALALAASPGLY
5   THRLEUSERLEUTHRGLYTHRASPLEUGLU
6   METGLUMETVALALAARGVALALALEUVAL
7   GLNPROHISGLUPROGLYALATHRTHRVAL
8   PROALAARGLYSPHEPHEASPILECYSARG
9   GLYLEUPROGLUGLYALAGLUILEALAVAL
10   GLNLEUGLUGLYGLUARGMETLEUVALARG
11   SERGLYARGSERARGPHESERLEUSERTHR
12   LEUPROALAALAASPPHEPROASNLEUASP
13   ASPTRPGLNSERGLUVALGLUPHETHRLEU
14   PROGLNALATHRMETLYSARGLEUILEGLU
15   ALATHRGLNPHESERMETALAHISGLNASP
16   VALARGTYRTYRLEUASNGLYMETLEUPHE
17   GLUTHRGLUGLYGLUGLULEUARGTHRVAL
18   ALATHRASPGLYHISARGLEUALAVALCYS
19   SERMETPROILEGLYGLNSERLEUPROSER
20   HISSERVALILEVALPROARGLYSGLYVAL
21   ILEGLULEUMETARGMETLEUASPGLYGLY
22   ASPASNPROLEUARGVALGLNILEGLYSER
23   ASNASNILEARGALAHISVALGLYASPPHE
24   ILEPHETHRSERLYSLEUVALASPGLYARG
25   PHEPROASPTYRARGARGVALLEUPROLYS
26   ASNPROASPLYSHISLEUGLUALAGLYCYS
27   ASPLEULEULYSGLNALAPHEALAARGALA
28   ALAILELEUSERASNGLULYSPHEARGGLY
29   VALARGLEUTYRVALSERGLUASNGLNLEU
30   LYSILETHRALAASNASNPROGLUGLNGLU
31   GLUALAGLUGLUILELEUASPVALTHRTYR
32   SERGLYALAGLUMETGLUILEGLYPHEASN
33   VALSERTYRVALLEUASPVALLEUASNALA
34   LEULYSCYSGLUASNVALARGMETMETLEU
35   THRASPSERVALSERSERVALGLNILEGLU
36   ASPALAALASERGLNSERALAALATYRVAL
37   VALMETPROMETARGLEU

Samples:

sample_1: The E. coli b-clamp, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 0.45 mM

sample_conditions_1: temperature: 308.15 K

sample_conditions_2: temperature: 313.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
4D 1H-13C HMQC NOSEY- HMQC NOSEYsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Agilent VNMRS 800 MHz