BMRB Entry 51432
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51432
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Title: Bcl-xl-delC UV exposed assigned chemical shifts used for TALOS calculation PubMed: 36597005
Deposition date: 2022-05-06 Original release date: 2022-05-17
Authors: Mishra, Pushpa
Citation: Bera, Akash; Singh, Suraj; D'Souza, Jacinta; Hosur, Ramakrishna; Mishra, Pushpa. "Effect of UV Stress on the Structure and Function of Pro-apoptotic Bid and Anti-apoptotic Bcl-xl proteins" Chembiochem ., e202200682-e202200682 (2023).
Assembly members:
entity_1, polymer, 171 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
entity_1: MSQSNRELVVDFLSYKLSQK
GYSWSQFSDVEENRTEAPEG
TESEAVKQALREAGDEFELR
YRRAFSDLTSQLHITPGTAY
QSFEQVVNELFRDGVNWGRI
VAFFSFGGALCVESVDKEMQ
VLVSRIAAWMATYLNDHLEP
WIQENGGWDTFVELYGNNAA
AESRKGQERLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 42 |
| 15N chemical shifts | 60 |
| 1H chemical shifts | 60 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bcl-xl | 1 |
Entities:
Entity 1, Bcl-xl 171 residues - Formula weight is not available
| 1 | MET | SER | GLN | SER | ASN | ARG | GLU | LEU | VAL | VAL | ||||
| 2 | ASP | PHE | LEU | SER | TYR | LYS | LEU | SER | GLN | LYS | ||||
| 3 | GLY | TYR | SER | TRP | SER | GLN | PHE | SER | ASP | VAL | ||||
| 4 | GLU | GLU | ASN | ARG | THR | GLU | ALA | PRO | GLU | GLY | ||||
| 5 | THR | GLU | SER | GLU | ALA | VAL | LYS | GLN | ALA | LEU | ||||
| 6 | ARG | GLU | ALA | GLY | ASP | GLU | PHE | GLU | LEU | ARG | ||||
| 7 | TYR | ARG | ARG | ALA | PHE | SER | ASP | LEU | THR | SER | ||||
| 8 | GLN | LEU | HIS | ILE | THR | PRO | GLY | THR | ALA | TYR | ||||
| 9 | GLN | SER | PHE | GLU | GLN | VAL | VAL | ASN | GLU | LEU | ||||
| 10 | PHE | ARG | ASP | GLY | VAL | ASN | TRP | GLY | ARG | ILE | ||||
| 11 | VAL | ALA | PHE | PHE | SER | PHE | GLY | GLY | ALA | LEU | ||||
| 12 | CYS | VAL | GLU | SER | VAL | ASP | LYS | GLU | MET | GLN | ||||
| 13 | VAL | LEU | VAL | SER | ARG | ILE | ALA | ALA | TRP | MET | ||||
| 14 | ALA | THR | TYR | LEU | ASN | ASP | HIS | LEU | GLU | PRO | ||||
| 15 | TRP | ILE | GLN | GLU | ASN | GLY | GLY | TRP | ASP | THR | ||||
| 16 | PHE | VAL | GLU | LEU | TYR | GLY | ASN | ASN | ALA | ALA | ||||
| 17 | ALA | GLU | SER | ARG | LYS | GLY | GLN | GLU | ARG | LEU | ||||
| 18 | GLU |
Samples:
sample_1: Bcl-xl, [U-99% 15N], 200 uM; Bcl-xl, [U-99% 13C; U-99% 15N], 400 uM; sodium phosphate 20 mM; DTT 2 mM; EDTA 1 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - data analysis
CARA - chemical shift assignment
TOPSPIN v3.6.3 - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts