BMRB Entry 51441
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51441
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Title: Solution NMR backbone chemical shift assignment for Tetrahymena Ctc1 OB-A domain PubMed: 35831498
Deposition date: 2022-05-13 Original release date: 2022-07-13
Authors: He, Yao; Song, He; Chan, Henry; Wang, Yaqiang; Liu, Baocheng; Susac, Lukas; Zhou, Hong; Feigon, Juli
Citation: He, Yao; Song, He; Chan, Henry; Liu, Baocheng; Wang, Yaqiang; Susac, Lukas; Zhou, Hong; Feigon, Juli. "Structure of Tetrahymena telomerase-bound CST with polymerase a-primase" Nature 608, 813-818 (2022).
Assembly members:
entity_1, polymer, 183 residues, Formula weight is not available
Natural source: Common Name: Tetrahymena thermophila Taxonomy ID: 5911 Superkingdom: Eukaryota Kingdom: not available Genus/species: Tetrahymena thermophila
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETduet
Entity Sequences (FASTA):
entity_1: MEIEEDLNLKILEDVKKLYL
QSFDYIKNGISSSLPSDKKF
LADDDIDLSRITFLYKFISV
NPTLLLINEKTQAKRRIFQG
EYLYGKKKIQFNIIAKNLEI
ERELIQFFKKPYQCYIMHNV
QVFQMLNKNKNNNVVEFMDS
EDLQSSVDCQLYYLIDESSH
VLEDDSMDFISTLTRLSDSF
NSN
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 164 |
| 1H chemical shifts | 164 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tetrahymena Ctc1 | 1 |
Entities:
Entity 1, Tetrahymena Ctc1 183 residues - Formula weight is not available
| 1 | MET | GLU | ILE | GLU | GLU | ASP | LEU | ASN | LEU | LYS | ||||
| 2 | ILE | LEU | GLU | ASP | VAL | LYS | LYS | LEU | TYR | LEU | ||||
| 3 | GLN | SER | PHE | ASP | TYR | ILE | LYS | ASN | GLY | ILE | ||||
| 4 | SER | SER | SER | LEU | PRO | SER | ASP | LYS | LYS | PHE | ||||
| 5 | LEU | ALA | ASP | ASP | ASP | ILE | ASP | LEU | SER | ARG | ||||
| 6 | ILE | THR | PHE | LEU | TYR | LYS | PHE | ILE | SER | VAL | ||||
| 7 | ASN | PRO | THR | LEU | LEU | LEU | ILE | ASN | GLU | LYS | ||||
| 8 | THR | GLN | ALA | LYS | ARG | ARG | ILE | PHE | GLN | GLY | ||||
| 9 | GLU | TYR | LEU | TYR | GLY | LYS | LYS | LYS | ILE | GLN | ||||
| 10 | PHE | ASN | ILE | ILE | ALA | LYS | ASN | LEU | GLU | ILE | ||||
| 11 | GLU | ARG | GLU | LEU | ILE | GLN | PHE | PHE | LYS | LYS | ||||
| 12 | PRO | TYR | GLN | CYS | TYR | ILE | MET | HIS | ASN | VAL | ||||
| 13 | GLN | VAL | PHE | GLN | MET | LEU | ASN | LYS | ASN | LYS | ||||
| 14 | ASN | ASN | ASN | VAL | VAL | GLU | PHE | MET | ASP | SER | ||||
| 15 | GLU | ASP | LEU | GLN | SER | SER | VAL | ASP | CYS | GLN | ||||
| 16 | LEU | TYR | TYR | LEU | ILE | ASP | GLU | SER | SER | HIS | ||||
| 17 | VAL | LEU | GLU | ASP | ASP | SER | MET | ASP | PHE | ILE | ||||
| 18 | SER | THR | LEU | THR | ARG | LEU | SER | ASP | SER | PHE | ||||
| 19 | ASN | SER | ASN |
Samples:
sample_1: Tetrahymena Ctc1 OB-A domain, [U-13C; U-15N; U-2H], 0.8 mM; TRIS 20 mM; sodium chloride 50 mM; TCEP 1 mM; sodium azide 3 mM
sample_conditions_1: ionic strength: 0.07 M; pH: 7.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY v1.414 - chemical shift assignment
TOPSPIN v4.1 - collection, processing
TOPSPIN v3.5 - collection, processing
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts