BMRB Entry 51443
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51443
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Title: Solution NMR backbone chemical shift assignment for Tetrahymena p50 peptide PubMed: 35831498
Deposition date: 2022-05-14 Original release date: 2022-07-13
Authors: He, Yao; Song, He; Chan, Henry; Wang, Yaqiang; Liu, Baocheng; Susac, Lukas; Zhou, Hong; Feigon, Juli
Citation: He, Yao; Song, He; Chan, Henry; Liu, Baocheng; Wang, Yaqiang; Susac, Lukas; Zhou, Hong; Feigon, Juli. "Structure of Tetrahymena telomerase-bound CST with polymerase a-primase" Nature 608, 813-818 (2022).
Assembly members:
entity_1, polymer, 24 residues, Formula weight is not available
Natural source: Common Name: Tetrahymena thermophila Taxonomy ID: 5911 Superkingdom: Eukaryota Kingdom: not available Genus/species: Tetrahymena thermophila
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETduet
Entity Sequences (FASTA):
entity_1: GCQQDDFGDGCLLQIVNYTH
QSLK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 39 |
15N chemical shifts | 20 |
1H chemical shifts | 20 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Tetrahymena p50 | 1 |
Entities:
Entity 1, Tetrahymena p50 24 residues - Formula weight is not available
1 | GLY | CYS | GLN | GLN | ASP | ASP | PHE | GLY | ASP | GLY | ||||
2 | CYS | LEU | LEU | GLN | ILE | VAL | ASN | TYR | THR | HIS | ||||
3 | GLN | SER | LEU | LYS |
Samples:
sample_1: Tetrahymena p50 peptide, [U-13C; U-15N], 0.5 mM; TRIS 20 mM; sodium chloride 50 mM; sodium azide 3 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 0.07 M; pH: 7.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY v1.414 - chemical shift assignment
TOPSPIN v4.1 - collection, processing
TOPSPIN v3.5 - collection, processing
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
- Bruker AVANCE III HD 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts